Hemolysis and hematology profile during perfusion: inter-species comparison

Détails

ID Serval
serval:BIB_114C5095C8A8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Hemolysis and hematology profile during perfusion: inter-species comparison
Périodique
International Journal of Artificial Organs
Auteur(s)
Mueller  X. M., Tevaearai  H. T., Jegger  D., Tucker  O., von Segesser  L. K.
ISSN
0391-3988 (Print)
Statut éditorial
Publié
Date de publication
02/2001
Volume
24
Numéro
2
Pages
89-94
Notes
Journal Article --- Old month value: Feb
Résumé
INTRODUCTION: Cardiopulmonary bypass components need to be tested on an animal model before their clinical application. Because their weight is similar to that of man, the calf and pig are often used. This study compares the impact of prolonged perfusion on hemolysis and hematology profile in both species. METHODS: Three calves (mean bodyweight: 77.2+/-4.4 kg) and three pigs (80+/-5.3 kg) were connected to an extracorporeal circulation circuit by jugular venous and carotid arterial cannulation, with a mean flow rate of 3.5L/min for 6h. After 7 days, the animals were sacrificed. A standard battery of blood samples was taken before, throughout, and 24h, 48h and 7 days after bypass. ANOVA was used for repeated measurements. RESULTS: Absolute values of red cell count were higher in the calf (p<0.001), while normalized values were higher in the pig (p<0.001). Absolute values of white cell count were higher in the pig, while normalized values diverged toward the end of the perfusion with an increase in the calf and a decrease in the pig (p<0.001). Free plasma Hb and LDH exhibited similar profiles in both groups. CONCLUSIONS: In the setting of prolonged perfusion, species type--bovine or porcine--has an impact on hematology profile, but not on hemolytic parameters. These findings should be taken into account when cardiopulmonary bypass components are tested.
Mots-clé
Analysis of Variance Animals *Blood Cell Count *Blood Gas Analysis *Cardiopulmonary Bypass Cattle Hemodynamic Processes/physiology Hemolysis/*physiology Physiology, Comparative Swine
Pubmed
Web of science
Création de la notice
28/01/2008 10:28
Dernière modification de la notice
20/08/2019 13:38
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