Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem

Détails

ID Serval
serval:BIB_1141A7E64CA0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem
Périodique
Nature
Auteur⸱e⸱s
Beguin  P., Nagashima  K., Gonoi  T., Shibasaki  T., Takahashi  K., Kashima  Y., Ozaki  N., Geering  K., Iwanaga  T., Seino  S.
ISSN
0028-0836 (Print)
Statut éditorial
Publié
Date de publication
06/2001
Volume
411
Numéro
6838
Pages
701-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun 7
Résumé
Voltage-dependent calcium (Ca2+) channels are involved in many specialized cellular functions, and are controlled by intracellular signals such as heterotrimeric G-proteins, protein kinases and calmodulin (CaM). However, the direct role of small G-proteins in the regulation of Ca2+ channels is unclear. We report here that the GTP-bound form of kir/Gem, identified originally as a Ras-related small G-protein that binds CaM, inhibits high-voltage-activated Ca2+ channel activities by interacting directly with the beta-subunit. The reduced channel activities are due to a decrease in alpha1-subunit expression at the plasma membrane. The binding of Ca2+/CaM to kir/Gem is required for this inhibitory effect by promoting the cytoplasmic localization of kir/Gem. Inhibition of L-type Ca2+ channels by kir/Gem prevents Ca2+-triggered exocytosis in hormone-secreting cells. We propose that the small G-protein kir/Gem, interacting with beta-subunits, regulates Ca2+ channel expression at the cell surface.
Mots-clé
Animals Calcium/metabolism Calcium Channels/*metabolism Cell Line Cell Membrane/metabolism Exocytosis/physiology Guanosine Triphosphate/metabolism Immediate-Early Proteins/*metabolism Insulin/secretion Mice Models, Biological Monomeric GTP-Binding Proteins/*metabolism Protein Binding Recombinant Fusion Proteins/metabolism Two-Hybrid System Techniques Xenopus
Pubmed
Web of science
Création de la notice
24/01/2008 12:28
Dernière modification de la notice
20/08/2019 12:38
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