The beta1 and beta3 integrins promote T cell receptor-mediated cytotoxic T lymphocyte activation.

Détails

ID Serval
serval:BIB_11349B25E7E3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The beta1 and beta3 integrins promote T cell receptor-mediated cytotoxic T lymphocyte activation.
Périodique
Journal of Biological Chemistry
Auteur(s)
Doucey M.A., Legler D.F., Faroudi M., Boucheron N., Baumgaertner P., Naeher D., Cebecauer M., Hudrisier D., Rüegg C., Palmer E., Valitutti S., Bron C., Luescher I.F.
ISSN
0021-9258[print], 0021-9258[linking]
Statut éditorial
Publié
Date de publication
2003
Volume
278
Numéro
29
Pages
26983-26991
Langue
anglais
Résumé
Recognition by CD8+ cytotoxic T lymphocytes (CTLs) of antigenic peptides bound to major histocompatibility class (MHC) I molecules on target cells leads to sustained calcium mobilization and CTL degranulation resulting in perforin-dependent killing. We report that beta1 and beta3 integrin-mediated adhesion to extracellular matrix proteins on target cells and/or surfaces dramatically promotes CTL degranulation. CTLs, when adhered to fibronectin but not CTL in suspension, efficiently degranulate upon exposure to soluble MHC.peptide complexes, even monomeric ones. This adhesion induces recruitment and activation of the focal adhesion kinase Pyk2, the cytoskeleton linker paxillin, and the Src kinases Lck and Fyn in the contact site. The T cell receptor, by association with Pyk2, becomes part of this adhesion-induced activation cluster, which greatly increases its signaling.
Mots-clé
Animals, Antigens, CD29/metabolism, Antigens, CD8/metabolism, Calcium Signaling, Cell Adhesion, Cell Degranulation, Cell Line, Cytoskeletal Proteins/metabolism, Cytotoxicity, Immunologic, Fibronectins/metabolism, Focal Adhesion Kinase 2, H-2 Antigens/genetics, H-2 Antigens/metabolism, Integrin beta3/metabolism, Lymphocyte Activation, Lymphocyte Specific Protein Tyrosine Kinase p56(lck)/metabolism, Mice, Paxillin, Phosphoproteins/metabolism, Protein-Tyrosine Kinases/metabolism, Proto-Oncogene Proteins/metabolism, Proto-Oncogene Proteins c-fyn, Receptor-CD3 Complex, Antigen, T-Cell/metabolism, Receptors, Antigen, T-Cell/metabolism, Signal Transduction, T-Lymphocytes, Cytotoxic/cytology, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:19
Dernière modification de la notice
20/08/2019 13:38
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