Introduction: Post-mortem CT angiography is the method of choice for the post-mortem imaging investigations of the cardio-vascular (CV) system. However, autopsy still remains the gold standard for CV measurement. Nevertheless, there aren’t any studies on CV measurements on the multi-phase post-mortem angiography (MPMCTA) which includes comparisons with autopsy. Therefore, the aim of this study is to compare CV measurements between the native CT-scan and the three phases of the MPMCTA to find out which of these modalities correlate the best with autopsy measurements. Methods: For this study we selected retrospectively 50 post-mortem cases that underwent both MPMCTA and autopsy. A comparison was carried out between the CV measurements obtain with imaging (aorta; heart cavities and cardiac wall thicknesses; maximum cardiac diameter and cardiothoracic ratio) and at the autopsy (aorta; cardiac valves, ventricular thicknesses and weight). Results: Our results show that the dynamic phase displays an advantage for the measurement of the aortas. However, the MPMCTA is not accurate to measure the cardiac wall thicknesses. The measurements of the heart cavities show no correlation with the heart valves. The cardiothoracic ratio measured by the MPMCTA show no correlation with the heart weight. Nevertheless, the maximum cardiac diameter exhibits a correlation with the latter on the venous and dynamic phase. Conclusions: These results show that only few CV parameters measured with imaging correlate with measurement obtained at the autopsy. These results indicate that in order to better estimate values obtained at the autopsy, we need to define new reference values for the CV measurement on MPMCTA.