Lysis of tumor cells by the retargeting of murine cytolytic T lymphocytes with bispecific antibodies

Détails

ID Serval
serval:BIB_10DC66873026
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Lysis of tumor cells by the retargeting of murine cytolytic T lymphocytes with bispecific antibodies
Périodique
International Journal of Cancer
Auteur(s)
Barr  I. G., MacDonald  H. R., Buchegger  F., von Fliedner  V.
ISSN
0020-7136 (Print)
Statut éditorial
Publié
Date de publication
09/1987
Volume
40
Numéro
3
Pages
423-9
Notes
Journal Article --- Old month value: Sep 15
Résumé
The specificity and efficiency of tumor-cell lysis by the retargeting of murine cytolytic lymphocytes (CTL) with bispecific antibodies was examined. Bispecific antibodies (also known as heteroaggregated or hybrid antibodies) were produced by the chemical coupling of monoclonal antibodies (MAbs) against H-2 antigens and the murine T-cell receptor (TCR). Murine tumor cell lines which expressed on their plasma membrane an antigen reactive with one component of the bispecific antibody were efficiently lysed in the presence of polyclonal murine CTL. CTL capable of lysis were generated by stimulating spleen or lymph-node cells with ConA and IL-2, while unstimulated cells or cells incubated only with IL-2 showed no lysis of target cells with bispecific antibodies. Furthermore, the lysis of target cells by bispecific antibodies and CTL did not cause the lysis of bystander cells (cells not expressing an antigen recognized by the antibody which are mixed with the target cells). The efficient CTL-mediated lysis observed with these antibodies makes this a promising approach for the immunotherapy of human cancer.
Mots-clé
Animals Antibodies, Monoclonal/*immunology Antibody Specificity Cell Line Concanavalin A/pharmacology *Cytotoxicity, Immunologic Dose-Response Relationship, Immunologic H-2 Antigens/analysis/*immunology Interleukin-2 Mice Mice, Inbred BALB C Neoplasms, Experimental/*immunology Receptors, Antigen, T-Cell/*immunology T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 12:27
Dernière modification de la notice
20/08/2019 13:38
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