Association of hemolytic activity of Pseudomonas entomophila, a versatile soil bacterium, with cyclic lipopeptide production.

Détails

ID Serval
serval:BIB_10DA8D7C9A81
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Association of hemolytic activity of Pseudomonas entomophila, a versatile soil bacterium, with cyclic lipopeptide production.
Périodique
Applied and environmental microbiology
Auteur(s)
Vallet-Gely I., Novikov A., Augusto L., Liehl P., Bolbach G., Péchy-Tarr M., Cosson P., Keel C., Caroff M., Lemaitre B.
ISSN
1098-5336[electronic]
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
76
Numéro
3
Pages
910-921
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Pseudomonas entomophila is an entomopathogenic bacterium that is able to infect and kill Drosophila melanogaster upon ingestion. Its genome sequence suggests that it is a versatile soil bacterium closely related to Pseudomonas putida. The GacS/GacA two-component system plays a key role in P. entomophila pathogenicity, controlling many putative virulence factors and AprA, a secreted protease important to escape the fly immune response. P. entomophila secretes a strong diffusible hemolytic activity. Here, we showed that this activity is linked to the production of a new cyclic lipopeptide containing 14 amino acids and a 3-C(10)OH fatty acid that we called entolysin. Three nonribosomal peptide synthetases (EtlA, EtlB, EtlC) were identified as responsible for entolysin biosynthesis. Two additional components (EtlR, MacAB) are necessary for its production and secretion. The P. entomophila GacS/GacA two-component system regulates entolysin production, and we demonstrated that its functioning requires two small RNAs and two RsmA-like proteins. Finally, entolysin is required for swarming motility, as described for other lipopeptides, but it does not participate in the virulence of P. entomophila for Drosophila. While investigating the physiological role of entolysin, we also uncovered new phenotypes associated with P. entomophila, including strong biocontrol abilities.
Pubmed
Web of science
Open Access
Oui
Création de la notice
05/01/2010 15:18
Dernière modification de la notice
20/08/2019 13:38
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