Crescent bodies of Parachlamydia acanthamoeba and its life cycle within Acanthamoeba polyphaga: an electron micrograph study

Détails

ID Serval
serval:BIB_10C385E057A0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Crescent bodies of Parachlamydia acanthamoeba and its life cycle within Acanthamoeba polyphaga: an electron micrograph study
Périodique
Applied and Environmental Microbiology
Auteur(s)
Greub  G., Raoult  D.
ISSN
0099-2240 (Print)
Statut éditorial
Publié
Date de publication
06/2002
Volume
68
Numéro
6
Pages
3076-84
Notes
Journal Article --- Old month value: Jun
Résumé
Parachlamydiaceae are endosymbionts of free-living amoeba first identified in 1997. Two developmental stages, elementary and reticulate bodies, were observed; however, their localization and proportions according to culture condition and duration remain unknown. The life cycle of Parachlamydia acanthamoeba within Acanthamoeba polyphaga was studied by transmission electron microscopy of 8-, 36-, and 144-h coculture. Morphometry and quantification were performed using SAMBA software. The elementary body, the predominant stage within the amoebae, was located mainly within their vacuoles. The multiplication of Parachlamydia bacteria by binary fission of reticulate bodies was independently associated with culture in PYG broth (odds ratio [OR] = 4.4; 95% confidence interval [CI], 1.55 to 12.46) and with the presence of reticulate bodies within the amoebae (OR = 2.10; 95% CI, 1.53 to 2.89). A third developmental stage was observed, the crescent body. Its presence outside and inside the amoebae was associated mainly with prolonged incubation time (OR = 3.98; 95% CI, 1.49 to 10.68, and OR = 5.98; 95% CI, 1.75 to 20.4, respectively). Elementary and crescent bodies were released into the extracellular medium within vesicles or after amoebal lysis. For both, phagocytosis was their mode of entry. This electron micrograph study revealed another infective developmental stage, the crescent body, and provided quantitative analysis of the life cycle of P. acanthamoeba within A. polyphaga.
Mots-clé
Acanthamoeba/*microbiology/ultrastructure Animals Chlamydiales/physiology/*ultrastructure Life Cycle Stages Microscopy, Electron
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:27
Dernière modification de la notice
20/08/2019 13:38
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