Basolateral sorting of furin in MDCK cells requires a phenylalanine-isoleucine motif together with an acidic amino acid cluster.

Détails

ID Serval
serval:BIB_10486
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Basolateral sorting of furin in MDCK cells requires a phenylalanine-isoleucine motif together with an acidic amino acid cluster.
Périodique
Molecular and Cellular Biology
Auteur⸱e⸱s
Simmen T., Nobile M., Bonifacino J.S., Hunziker W.
ISSN
0270-7306
Statut éditorial
Publié
Date de publication
1999
Volume
19
Numéro
4
Pages
3136-3144
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Furin is a subtilisin-related endoprotease which processes a wide range of bioactive proteins. Furin is concentrated in the trans-Golgi network (TGN), where proteolytic activation of many precursor proteins takes place. A significant fraction of furin, however, cycles among the TGN, the plasma membrane, and endosomes, indicating that the accumulation in the TGN reflects a dynamic localization process. The cytosolic domain of furin is necessary and sufficient for TGN localization, and two signals are responsible for retrieval of furin to the TGN. A tyrosine-based (YKGL) motif mediates internalization of furin from the cell surface into endosomes. An acidic cluster that is part of two casein kinase II phosphorylation sites (SDSEEDE) is then responsible for retrieval of furin from endosomes to the TGN. In addition, the acidic EEDE sequence also mediates endocytic activity. Here, we analyzed the sorting of furin in polarized epithelial cells. We show that furin is delivered to the basolateral surface of MDCK cells, from where a significant fraction of the protein can return to the TGN. A phenylalanine-isoleucine motif together with the acidic EEDE cluster is required for basolateral sorting and constitutes a novel signal regulating intracellular traffic of furin.
Mots-clé
Amino Acid Sequence, Amino Acids, Dicarboxylic, Animals, Biological Transport, Cell Compartmentation, Cell Membrane/metabolism, Cell Polarity/physiology, Dogs, Endosomes/metabolism, Epithelial Cells/cytology, Furin, Golgi Apparatus/metabolism, Isoleucine, Kidney/cytology, Molecular Sequence Data, Phenylalanine, Phosphorylation, Recombinant Fusion Proteins/metabolism, Serine/metabolism, Subtilisins/genetics, Subtilisins/metabolism
Pubmed
Web of science
Création de la notice
19/11/2007 13:00
Dernière modification de la notice
20/08/2019 13:37
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