Functional characterization and receptor binding studies of the malic enzyme thyroid hormone response element.

Détails

ID Serval
serval:BIB_1041D990FE6E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Functional characterization and receptor binding studies of the malic enzyme thyroid hormone response element.
Périodique
Journal of Biological Chemistry
Auteur⸱e⸱s
Desvergne B., Petty K.J., Nikodem V.M.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
01/1991
Peer-reviewed
Oui
Volume
266
Numéro
2
Pages
1008-1013
Langue
anglais
Résumé
We previously showed that the 5'-flanking region of the malic enzyme (ME) gene contains a cis-regulatory element (-281 to -261) that binds thyroid hormone receptors and confers triiodothyronine (T3) inducibility of transcription to the ME promoter (Petty, K.J., Desvergne, B., Mitsuhashi, T., and Nikodem, V. M. (1990) J. Biol. Chem. 265, 7395-7400). In this report, we have used deletion and mutation analyses of the ME thyroid hormone response element (TRE) to evaluate the roles of several subregions of TRE in T3 binding and transactivation. ME TRE was shown to act as an enhancer conferring T3 responsiveness to a heterologous promoter thymidine kinase. Although T3 treatment induced the promoter activity, the absence of hormone resulted in repression as measured by the level of chloramphenicol acetyltransferase expression in the NIH 3T3 transient expression system in the presence of overexpressed receptor. The degree of repression was similar to the degree of T3 induction observed for the same TRE mutants. Mutation and deletion analyses indicated that the functional TRE is comprised of discrete regions that are not contiguous, with a dominant role of a cluster of G residues and an AGGACA sequence. Both functions, induction and repression of transcription, correlated with receptor binding to the ME TRE as determined by competition binding assays using wild type and mutated TRE as competitors.
Mots-clé
Animals, Base Sequence, Malate Dehydrogenase/genetics, Malate Dehydrogenase/metabolism, Male, Molecular Sequence Data, Mutation, Plasmids, Promoter Regions, Genetic, Rats, Receptors, Thyroid Hormone/metabolism, Thyroid Hormones/genetics, Thyroid Hormones/metabolism, Transcription, Genetic, Transfection
Pubmed
Web of science
Création de la notice
24/01/2008 16:27
Dernière modification de la notice
20/08/2019 13:37
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