The diversity of antigen-specific TCR repertoires reflects the relative complexity of epitopes recognized.

Détails

ID Serval
serval:BIB_1038
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The diversity of antigen-specific TCR repertoires reflects the relative complexity of epitopes recognized.
Périodique
Human Immunology
Auteur⸱e⸱s
Maryanski J.L., Casanova J.L., Falk K., Gournier H., Jaulin C., Kourilsky P., Lemonnier F.A., Lüthy R., Rammensee H.G., Rötzschke O., Servis C., López J.A.
ISSN
0198-8859
Statut éditorial
Publié
Date de publication
1997
Volume
54
Numéro
2
Pages
117-128
Langue
anglais
Notes
Publication types: Journal Article ; Review Publication Status: ppublish
Résumé
Antigen-selected T cell receptor (TCR) repertoires vary in complexity from very limited to extremely diverse. We have previously characterized two different CD8 T cell responses, which are restricted by the same mouse major histocompatibility complex (MHC) class I molecule, H-2 Kd. The TCR repertoire in the response against a determinant from Plasmodium berghei circumsporozoite protein (PbCS; region 252-260) is very diverse, whereas TCRs expressed by clones specific for a determinant in region 170-179 of HLA-CW3 (human) MHC class I molecule show relatively limited structural diversity. We had already demonstrated that cytolytic T lymphocyte (CTL) clones specific for the PbCS peptide display diverse patterns of antigen recognition when tested with a series of single Ala-substituted PbCS peptides or mutant. H-2 Kd molecules. We now show that CW3-specific CTL clones display much less diverse patterns of recognition. Our earlier functional studies with synthetic peptide variants suggested that the optimal peptides recognized were 9 (or 8) residues long for PbCS and 10 residues long for CW3. We now present more direct evidence that the natural CW3 ligand is indeed a 10-mer. Our functional data together with molecular modeling suggest that the limited TCR repertoire selected during the CW3 response is not due to a paucity of available epitopes displayed at the surface of the CW3 peptide/Kd complex. We discuss other factors, such as the expression of similar self MHC peptide sequences, that might be involved in trimming this TCR repertoire.
Mots-clé
Animals, Antigen Presentation, Cytotoxicity, Immunologic, Epitopes/genetics, Epitopes/immunology, H-2 Antigens/immunology, HLA Antigens/immunology, Histocompatibility Antigens Class I/immunology, Humans, Mice, Models, Immunological, Peptides/immunology, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell/immunology, Recombinant Proteins/immunology, T-Lymphocyte Subsets/immunology
Pubmed
Web of science
Création de la notice
19/11/2007 12:00
Dernière modification de la notice
20/08/2019 12:37
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