SIgA-Shigella Immune Complexes Interact with Dectin-1 and SIGNR3 to Differentially Regulate Mouse Peyer's Patch and Mesenteric Lymph Node Dendritic Cell's Responsiveness.

Détails

ID Serval
serval:BIB_1013185D5FAB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
SIgA-Shigella Immune Complexes Interact with Dectin-1 and SIGNR3 to Differentially Regulate Mouse Peyer's Patch and Mesenteric Lymph Node Dendritic Cell's Responsiveness.
Périodique
Journal of molecular biology
Auteur(s)
Mikulic J., Bioley G., Corthésy B.
ISSN
1089-8638 (Electronic)
ISSN-L
0022-2836
Statut éditorial
Publié
Date de publication
21/07/2017
Peer-reviewed
Oui
Volume
429
Numéro
15
Pages
2387-2400
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
In addition to contributing to immune exclusion at mucosal surfaces, secretory IgA (SIgA) made of polymeric IgA and secretory component is able to selectively reenter via microfold cells into Peyer's patches (PPs) present along the intestine and to associate with dendritic cells (DCs) of the CD11c+CD11b+MHCII+F4/80-CD8-phenotype in the subepithelial dome region and the draining mesenteric lymph nodes (MLNs). However, the nature of the receptor(s) for SIgA on murine PP and MLN DCs is unknown. We find that glycosylated secretory component moiety and polymeric IgA are both involved in the specific interaction with these cells. Using blocking antibodies and competition experiments, we identify Dectin-1 and specific intercellular adhesion molecule-3 grabbing non-integrin receptor 3 (SIGNR3) as receptors for SIgA. While SIgA-commensal immune complexes (ICs) contribute to local homeostasis upon interaction with mucosal DCs, the picture is less clear for pathogenic agents. We find that in comparison with incubation of Shigella flexneri alone, association of the enteropathogen with SIgA prompts freshly isolated DCs from PPs and MLNs to invert the production of pro- versus non-inflammatory cytokines/chemokines. The sum of the data suggests that in contrast to IgG-based ICs boosting immune reactivity of antigen-presenting cells, SIgA produced during an ongoing immune response can, in addition to its known function of immune exclusion, modulate mucosal DC conditioning via specific interaction with Dectin-1 and SIGNR3.

Mots-clé
Animals, Antibodies, Bacterial/metabolism, Antigen-Antibody Complex/metabolism, Antigens, Bacterial/metabolism, Antigens, CD/metabolism, Cytokines/metabolism, Dendritic Cells/immunology, Immunoglobulin A, Secretory/metabolism, Lectins, C-Type/metabolism, Lymph Nodes/immunology, Mice, Peyer's Patches/immunology, Shigella flexneri/immunology, Dectin-1, SIGNR3, cytokines, dendritic cells, secretory IgA
Pubmed
Web of science
Création de la notice
11/12/2017 11:59
Dernière modification de la notice
20/08/2019 13:36
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