Evaluation of a lysis-centrifugation system for recovery of yeasts and filamentous fungi from blood

Détails

ID Serval
serval:BIB_0FBF19F31250
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Evaluation of a lysis-centrifugation system for recovery of yeasts and filamentous fungi from blood
Périodique
Journal of Clinical Microbiology
Auteur(s)
Bille  J., Stockman  L., Roberts  G. D., Horstmeier  C. D., Ilstrup  D. M.
ISSN
0095-1137
Statut éditorial
Publié
Date de publication
09/1983
Peer-reviewed
Oui
Volume
18
Numéro
3
Pages
469-71
Notes
Journal Article --- Old month value: Sep
Résumé
A lysis-centrifugation system (Isolator; E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) was compared with a biphasic brain heart infusion (BHI) medium in a prospective study of 5,125 fungal blood cultures. The Isolator recovered 90.3% of the positive cultures, compared with 63.4% recovered by the biphasic BHI medium. Overall, the detection of fungemia was increased 36.6% by the Isolator. Mean recovery times for yeasts were 2.12 and 4.90 days for the Isolator and BHI bottles, respectively. Cultures of Histoplasma capsulatum required 8.0 and 24.14 days for recovery by the Isolator and BHI bottles, respectively. The Isolator provided a more rapid and sensitive means of detecting organisms associated with fungemia.
Mots-clé
Blood/*microbiology Centrifugation/methods Culture Media Evaluation Studies as Topic Fungi/*isolation & purification Humans Mycoses/*microbiology Yeasts/isolation & purification
Pubmed
Web of science
Création de la notice
11/02/2008 12:39
Dernière modification de la notice
20/08/2019 12:36
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