Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody
Détails
ID Serval
serval:BIB_0F9C4B470B97
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody
Périodique
European Journal of Immunology
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
02/1992
Volume
22
Numéro
2
Pages
399-404
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
A monoclonal antibody, B20.1, was generated by fusing spleen cells from a Lou rat immunized with a soluble alpha/beta T cell receptor (TcR; V alpha 2/V beta 2) to mouse myeloma cells. Analysis of a panel of V alpha 2 mRNA-expressing T cell lines, hybridomas and transfectants revealed that the B20.1 antibody was specific for murine TcR V alpha 2 chains. The V alpha 2+ T cell population was examined in various inbred strains by two-color immunofluorescence using B20.1 and CD4- and CD8-specific antibodies with the following results: (a) the B20.1 antibody detected most members of the TcR V alpha 2 subfamily in the four TcR V alpha haplotypes tested; (b) in most strains examined, TcR V alpha 2 expression was biased to the CD4 subset (7.4%-17.4% V alpha 2+ T cells) as compared to the CD8 compartment (3.8%-13.3%); (c) TcR V alpha 2 expression was not influenced by Mls gene products and (d) increased positive selection of V alpha 2+ CD8+ T cells by H-2k major histocompatibility complex molecules occurred in all murine strains tested of the TcR V alpha a, but not in those bearing the TcR V alpha b haplotype.
Mots-clé
Animals
Antibodies, Monoclonal/immunology
Antigens, CD4/analysis
Antigens, CD8/*metabolism
Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
Genes, MHC Class I
H-2 Antigens/*genetics
Haplotypes
Hybridomas
Mice
Receptors, Antigen, T-Cell, alpha-beta/immunology/*metabolism
Receptors, Antigen, T-Cell, gamma-delta/genetics
T-Lymphocyte Subsets/*immunology
Pubmed
Web of science
Création de la notice
28/01/2008 12:33
Dernière modification de la notice
20/08/2019 13:36