Inflammatory malignant fibrous histiocytomas and dedifferentiated liposarcomas: histological review, genomic profile, and MDM2 and CDK4 status favour a single entity

Détails

ID Serval
serval:BIB_0F99DBB28062
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Inflammatory malignant fibrous histiocytomas and dedifferentiated liposarcomas: histological review, genomic profile, and MDM2 and CDK4 status favour a single entity
Périodique
Journal of Pathology
Auteur(s)
Coindre  J. M., Hostein  I., Maire  G., Derre  J., Guillou  L., Leroux  A., Ghnassia  J. P., Collin  F., Pedeutour  F., Aurias  A.
ISSN
0022-3417 (Print)
Statut éditorial
Publié
Date de publication
2004
Volume
203
Numéro
3
Pages
822-830
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Résumé
Inflammatory malignant fibrous histiocytoma (inflammatory MFH) is a very rare tumour that occurs most often in the retroperitoneum. So far, it has been considered to be a special subtype of MFH. As it is now widely accepted that most retroperitoneal pleomorphic MFHs are dedifferentiated liposarcomas, the present study compared histological features, genomic profile (CGH analysis), and MDM2 and CDK4 status (immunohistochemistry, FISH, and quantitative PCR) in inflammatory MFHs from 12 patients and dedifferentiated liposarcomas that had an inflammatory MFH component from eight patients. Metaphase cytogenetic and FISH analyses were also performed on one inflammatory MFH. Histological review showed areas of well-differentiated liposarcoma in nine inflammatory MFHs. CGH analysis showed 12q13-15 amplification or gain in six of seven inflammatory MFHs and in seven of seven dedifferentiated liposarcomas. Immunohistochemistry showed positivity of tumour cells for MDM2 in every tumour in both groups and for CDK4 in ten and seven inflammatory MFHs and dedifferentiated liposarcomas, respectively. Metaphase cytogenetic and FISH analysis performed on one inflammatory MFH showed the presence of a supernumerary large marker chromosome and ring chromosome with high-level amplification of both MDM2 and CDK4 genes. FISH analysis on paraffin wax-embedded sections showed amplifications of MDM2 and CDK4 in seven of seven inflammatory MFHs and in seven of seven dedifferentiated liposarcomas. Quantitative PCR showed amplification of MDM2 in six and of CDK4 in seven of nine inflammatory MFHs. In conclusion, this study strongly suggests that most so-called inflammatory MFHs are dedifferentiated liposarcomas
Mots-clé
Adult/Aged/Aged,80 and over/Cell Differentiation/Cyclin-Dependent Kinase 4/Cyclin-Dependent Kinases/genetics/metabolism/Female/Histiocytoma,Benign Fibrous/Pathology/Humans/Immunoenzyme Techniques/In Situ Hybridization,Fluorescence/Karyotyping/Liposarcoma/Male/Middle Aged/Neoplasm Proteins/Nuclear Proteins/Nucleic Acid Hybridization/Proto-Oncogene Proteins/Proto-Oncogene Proteins c-mdm2/Retroperitoneal Neoplasms
Pubmed
Web of science
Création de la notice
29/01/2008 18:35
Dernière modification de la notice
20/08/2019 12:36
Données d'usage