Polymorphic microsatellite markers were developed to study population structure and mating patterns of the monocarpic herb <i>Moricandia moricandioides</i> (Brassicaceae).
Illumina MiSeq sequencing was used to develop a panel of 15 polymorphic microsatellite markers that were tested across 77 individuals from three populations on the Iberian Peninsula. All markers were polymorphic in at least two studied populations, and the number of alleles ranged from one to 11 per locus. The levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.153 to 0.865, respectively. Nine and 11 loci were successfully amplified in the congeneric species <i>M. arvensis</i> and <i>M. foetida</i> , respectively.
The 15 microsatellite markers will be useful for population genetic studies of the genus <i>Moricandia</i> . These markers will serve as a useful tool for exploring population structure and mating patterns of <i>M. moricandioides</i> .