Targeting mTORC2 inhibits colon cancer cell proliferation in vitro and tumor formation in vivo.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_0F56871EBA25
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Targeting mTORC2 inhibits colon cancer cell proliferation in vitro and tumor formation in vivo.
Périodique
Molecular Cancer
Auteur(s)
Roulin D., Cerantola Y., Dormond-Meuwly A., Demartines N., Dormond O.
ISSN
1476-4598 (Electronic)
ISSN-L
1476-4598
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
9
Pages
57
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: epublish
Résumé
The mammalian target of rapamycin (mTOR), which exists in two functionally distinct complexes, mTORC1 and mTORC2 plays an important role in tumor growth. Whereas the role of mTORC1 has been well characterized in this process, little is known about the functions of mTORC2 in cancer progression. In this study, we explored the specific role of mTORC2 in colon cancer using a short hairpin RNA expression system to silence the mTORC2-associated protein rictor. We found that downregulation of rictor in HT29 and LS174T colon cancer cells significantly reduced cell proliferation. Knockdown of rictor also resulted in a G1 arrest as observed by cell cycle analysis. We further observed that LS174T cells deficient for rictor failed to form tumors in a nude mice xenograft model. Taken together, these results show that the inhibition of mTORC2 reduces colon cancer cell proliferation in vitro and tumor xenograft formation in vivo. They also suggest that specifically targeting mTORC2 may provide a novel treatment strategy for colorectal cancer.
Mots-clé
Adaptor Proteins, Signal Transducing, Animals, Carrier Proteins/metabolism, Cell Line, Tumor, Cell Proliferation, Colonic Neoplasms/metabolism, Colonic Neoplasms/pathology, Down-Regulation, Gene Knockdown Techniques, Humans, Mice, Proteins/metabolism, Transcription Factors/metabolism, Xenograft Model Antitumor Assays
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/06/2010 17:11
Dernière modification de la notice
13/05/2020 13:28
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