Quantitative proton spectroscopic imaging of the neurochemical profile in rat brain with microliter resolution at ultra-short echo times.

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_0F5307B9FF15
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Quantitative proton spectroscopic imaging of the neurochemical profile in rat brain with microliter resolution at ultra-short echo times.
Périodique
Magnetic Resonance in Medicine
Auteur(s)
Mlynárik V., Kohler I., Gambarota G., Vaslin A., Clarke P.G., Gruetter R.
ISSN
0740-3194 (Print)
ISSN-L
0740-3194
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
59
Numéro
1
Pages
52-58
Langue
anglais
Résumé
Proton spectroscopy allows the simultaneous quantification of a high number of metabolite concentrations termed the neurochemical profile. The spin echo full intensity acquired localization (SPECIAL) scheme with an echo time of 2.7 ms was used at 9.4T for excitation of a slab parallel to a home-built quadrature surface coil in conjunction with phase encoding in the two remaining spatial dimensions to yield an effective spatial resolution of 1.7 microL. The absolute concentrations of at least 10 metabolites were calculated from the spectra of individual voxels using LCModel analysis. The calculated concentrations were used for constructing quantitative metabolic maps of the neurochemical profile in normal and pathological rat brain. Summation of individual spectra was used to assess the neurochemical profile of unique brain regions, such as corpus callosum, in rat for the first time. Following focal ischemia in rat pups, imaging the neurochemical profile indicated increased choline groups in the ischemic core and increased glutamine in the penumbra, which is proposed to reflect glutamate excitotoxicity. We conclude that it is feasible to achieve a sensitivity that is sufficient for quantitative mapping of the neurochemical profile at microliter spatial resolution.
Mots-clé
Animals, Brain/metabolism, Female, Infarction, Middle Cerebral Artery/metabolism, Magnetic Resonance Spectroscopy/methods, Protons, Rats, Rats, Sprague-Dawley, Sensitivity and Specificity
Pubmed
Web of science
Open Access
Oui
Création de la notice
20/01/2008 18:48
Dernière modification de la notice
20/08/2019 13:36
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