GDNF and NGF released by synthetic guidance channels support sciatic nerve regeneration across a long gap

Détails

ID Serval
serval:BIB_0F43A1516F83
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
GDNF and NGF released by synthetic guidance channels support sciatic nerve regeneration across a long gap
Périodique
European Journal of Neuroscience
Auteur(s)
Fine  E. G., Decosterd  I., Papaloizos  M., Zurn  A. D., Aebischer  P.
ISSN
0953-816X (Print)
Statut éditorial
Publié
Date de publication
02/2002
Volume
15
Numéro
4
Pages
589-601
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
The present work was performed to determine the ability of neurotrophic factors to allow axonal regeneration across a 15-mm-long gap in the rat sciatic nerve. Synthetic nerve guidance channels slowly releasing NGF and GDNF were fabricated and sutured to the cut ends of the nerve to bridge the gap. After 7 weeks, nerve cables had formed in nine out of ten channels in both the NGF and GDNF groups, while no neuronal cables were present in the control group. The average number of myelinated axons at the midpoint of the regenerated nerves was significantly greater in the presence of GDNF than NGF (4942 +/-1627 vs. 1199 +/-431, P < or = 0.04). A significantly greater number of neuronal cells in the GDNF group, when compared to the NGF group, retrogradely transported FluoroGold injected distal to the injury site before explantation. The total number of labelled motoneurons observed in the ventral horn of the spinal cord was 98.1 +/-23.4 vs. 20.0 +/-8.5 (P < or = 0.001) in the presence of GDNF and NGF, respectively. In the dorsal root ganglia, 22.7% +/- 4.9% vs. 3.2% +/-1.9% (P +/-0.005) of sensory neurons were labelled retrogradely in the GDNF and NGF treatment groups, respectively. The present study demonstrates that, sustained delivery of GDNF and NGF to the injury site, by synthetic nerve guidance channels, allows regeneration of both sensory and motor axons over long gaps; GDNF leads to better overall regeneration in the sciatic nerve.
Mots-clé
Animals Axons/*drug effects/metabolism/ultrastructure Chick Embryo Ganglia, Spinal/cytology/drug effects/growth & development Glial Cell Line-Derived Neurotrophic Factor Male Motor Neurons/drug effects/metabolism/ultrastructure Nerve Fibers/drug effects/metabolism/ultrastructure Nerve Fibers, Myelinated/drug effects/metabolism/ultrastructure Nerve Growth Factor/metabolism/*pharmacology *Nerve Growth Factors Nerve Regeneration/*drug effects/physiology Nerve Tissue Proteins/metabolism/*pharmacology Neurons, Afferent/drug effects/metabolism/ultrastructure *Prostheses and Implants Rats Rats, Wistar Sciatic Nerve/*drug effects/*injuries/surgery
Pubmed
Web of science
Création de la notice
28/01/2008 10:45
Dernière modification de la notice
20/08/2019 12:36
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