Interleukin-1β Maturation Triggers Its Relocation to the Plasma Membrane for Gasdermin-D-Dependent and -Independent Secretion.

Détails

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_0F426EF76306
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Interleukin-1β Maturation Triggers Its Relocation to the Plasma Membrane for Gasdermin-D-Dependent and -Independent Secretion.
Périodique
Cell Reports
Auteur(s)
Monteleone M., Stanley A.C., Chen K.W., Brown D.L., Bezbradica J.S., von Pein J.B., Holley C.L., Boucher D., Shakespear M.R., Kapetanovic R., Rolfes V., Sweet M.J., Stow J.L., Schroder K.
ISSN
2211-1247 (Electronic)
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
24
Numéro
6
Pages
1425-1433
Langue
anglais
Résumé
IL-1β requires processing by caspase-1 to generate the active, pro-inflammatory cytokine. Acute IL-1β secretion from inflammasome-activated macrophages requires caspase-1-dependent GSDMD cleavage, which also induces pyroptosis. Mechanisms of IL-1β secretion by pyroptotic and non-pyroptotic cells, and the precise functions of caspase-1 and GSDMD therein, are unresolved. Here, we show that, while efficient early secretion of endogenous IL-1β from primary non-pyroptotic myeloid cells in vitro requires GSDMD, later IL-1β release in vitro and in vivo proceeds independently of GSDMD. IL-1β maturation is sufficient for slow, caspase-1/GSDMD-independent secretion of ectopic IL-1β from resting, non-pyroptotic macrophages, but the speed of IL-1β release is boosted by inflammasome activation, via caspase-1 and GSDMD. IL-1β cleavage induces IL-1β enrichment at PIP2-enriched plasma membrane ruffles, and this is a prerequisite for IL-1β secretion and is mediated by a polybasic motif within the cytokine. We thus reveal a mechanism in which maturation-induced IL-1β trafficking facilitates its unconventional secretion.
Mots-clé
caspase-1, gasdermin, inflammasome, interleukin-1, macrophage, neutrophil, phosphoinositides, pyroptosis, trafficking, unconventional protein secretion
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/08/2018 7:46
Dernière modification de la notice
20/08/2019 13:36
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