Biofilm formation by staphylococci on fresh, fresh-frozen and processed human and bovine bone grafts.

Détails

ID Serval
serval:BIB_0F3269450E73
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Biofilm formation by staphylococci on fresh, fresh-frozen and processed human and bovine bone grafts.
Périodique
European Cells and Materials
Auteur(s)
Clauss M., Tafin U.F., Bizzini A., Trampuz A., Ilchmann T.
ISSN
1473-2262 (Electronic)
ISSN-L
1473-2262
Statut éditorial
Publié
Date de publication
2013
Volume
25
Pages
159-166
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: epublish
Résumé
Biofilm formation is a multi-step process influenced by surface properties. We investigated early and mature biofilm of Staphylococcus aureus on 4 different biological calcium phosphate (CaP) bone grafts used for filling bone defects. We investigated standardised cylinders of fresh and fresh-frozen human bone grafts were harvested from femoral heads; processed humanand bovine bone grafts were obtained preformed. Biofilm formation was done in tryptic soy broth (TSB) using S. aureus (ATCC 29213) with static conditions. Biofilm density after 3 h (early biofilm) and 24 h (mature biofilm) was investigated by sonication and microcalorimetry. After 3 h, bacterial density was highest on fresh-frozenandfresh bone grafts. After 24 h, biofilm density was lowest on freshbone grafts (p < 0.001) compared to the other 3 materials, which did not differ quantitatively (p > 0.05). The lowest increase in bacterial density was detected on fresh bone grafts (p < 0.001). Despite normal shaped colonies, we found additional small colonies on the surface of the fresh and fresh-frozen samples by sonication. This was also apparent in microcalorimetric heat-flow curves. The four investigated CaP bone grafts showed minor structural differences in architecture but marked differences concerning serum coverage and the content of bone marrow, fibrous tissue and bone cells. These variations resulted in a decreased biofilm density on freshand fresh-frozenbone grafts after 24 h, despite an increased early biofilm formation and might also be responsible for the variations in colony morphology (small colonies). Detection of small colony variants by microcalorimetry might be a new approach to improve the understanding of biofilm formation.
Mots-clé
Aged, Animals, Bacterial Load, Biofilms, Bone Transplantation, Cattle, Cryopreservation, Femur Head/microbiology, Humans, Male, Sonication, Staphylococcus aureus/physiology
Pubmed
Web of science
Création de la notice
04/07/2013 20:52
Dernière modification de la notice
20/08/2019 13:36
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