Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo.

Détails

ID Serval
serval:BIB_0F1BE6ABB32E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Bovine herpesvirus 4 induces apoptosis of human carcinoma cell lines in vitro and in vivo.
Périodique
Cancer Research
Auteur(s)
Gillet L., Dewals B., Farnir F., de Leval L., Vanderplasschen A.
ISSN
0008-5472[print], 0008-5472[linking]
Statut éditorial
Publié
Date de publication
2005
Volume
65
Numéro
20
Pages
9463-9472
Langue
anglais
Résumé
The idea of using oncolytic viruses for the treatment of cancers was proposed a century ago. During the last two decades, viruses able to replicate specifically in cancer cells and to induce their lysis were identified and were genetically modified to improve their viro-oncolytic properties. More recently, a new approach consisting of inducing selective apoptosis in cancer cells through viral infection has been proposed; this approach has been called viro-oncoapoptosis. In the present study, we report the property of bovine herpesvirus-4 (BoHV-4) to induce, in vitro and in vivo, apoptosis of some human carcinomas. This conclusion relies on the following observations: (a) In vitro, BoHV-4 infection induced apoptosis of A549 and OVCAR carcinoma cell lines in a time- and dose-dependent manner. (b) Apoptosis was induced by the expression of an immediate-early or an early BoHV-4 gene, but did not require viral replication. (c) Cell treatment with caspase inhibitors showed that apoptosis induced by BoHV-4 relied mainly on caspase-10 activation. (d) Infection of cocultures of A549 or OVCAR cells mixed with human 293 cells (in which BoHV-4 does not induce apoptosis) showed that BoHV-4 specifically eradicated A549 or OVCAR cancer cells from the cocultures. (e) Finally, in vivo experiments done with nude mice showed that BoHV-4 intratumoral injections reduced drastically the growth of preestablished A549 xenografts. Taken together, these results suggest that BoHV-4 may have potential as a viro-oncoapoptotic agent for the treatment of some human carcinomas. Moreover, further identification of BoHV-4 proapoptotic gene(s) and the cellular pathways targeted by this or these gene(s) could lead to the design of new cancer therapeutic strategies.
Mots-clé
Animals, Apoptosis/physiology, Carcinoma/pathology, Carcinoma/therapy, Caspase 10, Caspases/metabolism, Cattle, Cell Line, Tumor, Coculture Techniques, Dogs, Female, Herpesvirus 4, Bovine/genetics, Herpesvirus 4, Bovine/metabolism, Humans, Xenograft Model Antitumor Assays
Pubmed
Open Access
Oui
Création de la notice
28/10/2010 10:39
Dernière modification de la notice
20/08/2019 13:35
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