Evidence for interaction between human PRUNE and nm23-H1 NDPKinase.

Détails

ID Serval
serval:BIB_0F037F13BF48
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Evidence for interaction between human PRUNE and nm23-H1 NDPKinase.
Périodique
Oncogene
Auteur(s)
Reymond A., Volorio S., Merla G., Al-Maghtheh M., Zuffardi O., Bulfone A., Ballabio A., Zollo M.
ISSN
0950-9232 (Print)
ISSN-L
0950-9232
Statut éditorial
Publié
Date de publication
1999
Volume
18
Numéro
51
Pages
7244-7252
Langue
anglais
Résumé
We have isolated a human and murine homologue of the Drosophila prune gene through dbEST searches. The gene is ubiquitously expressed in human adult tissues, while in mouse developing embryos a high level of expression is confined to the nervous system particularly in the dorsal root ganglia, cranial nerves, and neural retina. The gene is composed of eight exons and is located in the 1q21.3 chromosomal region. A pseudogene has been sequenced and mapped to chromosomal region 13q12. PRUNE protein retains the four characteristic domains of DHH phosphoesterases. The synergism between prune and awdK-pn in Drosophila has led various authors to propose an interaction between these genes. However, such an interaction has never been supported by biochemical data. By using interaction-mating and in vitro co-immunoprecipitation experiments, we show for the first time the ability of human PRUNE to interact with the human homologue of awd protein (nm23-H1). In contrast, PRUNE is impaired in its interaction with nm-23-H1-S120G mutant, a gain-of-function mutation associated with advanced neuroblastoma stages. Consistently, PRUNE and nm23-H1 proteins partially colocalize in the cytoplasm. The data presented are consistent with the view that PRUNE acts as a negative regulator of the nm23-H1 protein. We discuss how PRUNE regulates nm23-H1 protein and postulate possible implications of PRUNE in neuroblastoma progression.
Mots-clé
Adult, Amino Acid Sequence, Animals, Antigens, Neoplasm/genetics, Chromosome Mapping, Chromosomes, Human, Pair 13, Drosophila, Drosophila Proteins, Gene Expression Regulation, Gene Expression Regulation, Developmental, Humans, Insect Proteins/genetics, Mice, Molecular Sequence Data, Monomeric GTP-Binding Proteins, NM23 Nucleoside Diphosphate Kinases, Nucleoside-Diphosphate Kinase/genetics, Sequence Alignment, Sequence Homology, Amino Acid, Transcription Factors/genetics, Tumor Markers, Biological/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 16:52
Dernière modification de la notice
20/08/2019 13:35
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