Proteomic surfaceome analysis of mesothelioma.
Détails
ID Serval
serval:BIB_0ED8BD6D7B0E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Proteomic surfaceome analysis of mesothelioma.
Périodique
Lung Cancer
ISSN
1872-8332 (Electronic)
ISSN-L
0169-5002
Statut éditorial
Publié
Date de publication
2012
Volume
75
Numéro
2
Pages
189-196
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Résumé
Identification of new markers for malignant pleural mesothelioma (MPM) is a challenging clinical need. Here, we propose a quantitative proteomics primary screen of the cell surface exposed MPM N-glycoproteins, which provides the basis for the development of new protein-based diagnostic assays. Using the antibody-independent mass-spectrometry based cell surface capturing (CSC) technology, we specifically investigated the N-glycosylated surfaceome of MPM towards the identification of protein-marker candidates discriminatory between MPM and lung adenocarcinoma (ADCA). Relative quantitative CSC analysis of MPM cell line ZL55 in comparison with ADCA cell line Calu-3 revealed a bird's eye view of their respective surfaceomes. In a secondary screen of fifteen MPM and six ADCA, we used high throughput low density microarrays (LDAs) to verify specificity and sensitivity of nineteen N-glycoproteins overregulated in the surfaceome of MPM. This proteo-transcriptomic approach revealed thy-1/CD90 (THY1) and teneurin-2 (ODZ2) as protein-marker candidates for the discrimination of MPM from ADCA. Thy-1/CD90 was further validated by immunohistochemistry on frozen tissue sections of MPM and ADCA samples. Together, we present a combined proteomic and transcriptomic approach enabling the relative quantitative identification and pre-clinical selection of new MPM marker candidates.
Mots-clé
Adenocarcinoma/chemistry, Adult, Aged, Antigens, Thy-1/analysis, Blotting, Western, Cell Line, Tumor, Female, Humans, Immunohistochemistry, Lung Neoplasms/chemistry, Male, Membrane Proteins/analysis, Mesothelioma/chemistry, Middle Aged, Pleural Neoplasms/chemistry, Proteomics/methods
Pubmed
Web of science
Création de la notice
03/08/2014 13:38
Dernière modification de la notice
20/08/2019 12:35