Monitoring sulfate-reducing bacteria in heterotrophic biofilms.

Détails

ID Serval
serval:BIB_0ECE0AE5A194
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Monitoring sulfate-reducing bacteria in heterotrophic biofilms.
Périodique
Water Science and Technology
Auteur(s)
Power M., Araujo J.C., van der Meer J.R., Harms H., Wanner O.
ISSN
0273-1223
Statut éditorial
Publié
Date de publication
1999
Volume
39
Numéro
7
Pages
49-56
Langue
anglais
Résumé
To a laboratory reactor, in which heterotrophic biofilms were grown on stainless steel coupons under aerobic conditions, sulfate-reducing bacteria (SRB) were added in order to elucidate whether and how these microorganisms were going to establish themselves in the biofilm. Polymerase chain reaction for the dissimilatory sulfite reductase gene and in situ hybridization with probes directed against 16S ribosomal RNA were used to detect the SRB in the biofilm. Both methods proved to be suitable tools for monitoring the SRB in these experiments, which lasted seven days. In a first series of experiments, in which the SRB were added after a biofilm had already developed, the SRB could be detected only one day after addition. No evidence was found that the SRB penetrated the biofilm and established themselves in the anaerobic niches which were present. In a second series of experiments, in which the SRB were inoculated together with a seed of aerobic heterotrophtc microorganisms, the SRB were present in the biofilm over the whole biofilm depth and for the duration of the experiment. The study suggests that colonization of the steel coupons by the SRB added to the bulk fluid is hampered by the already developed biofilm, even though the heterogeneous biofilm structure and anaerobic zones in the biofilm depth offer the possibility for the SRB to penetrate and establish themselves.
Mots-clé
Biofilm, in situ hybridization, polymerase chain reaction, Robbins device, sulfate-reducing bacteria
Création de la notice
21/01/2008 14:35
Dernière modification de la notice
20/08/2019 13:35
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