Reactivity of murine and human recombinant LPS-binding protein (LBP) within LPS and gram negative bacteria

Détails

ID Serval
serval:BIB_0E89317AABBD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Reactivity of murine and human recombinant LPS-binding protein (LBP) within LPS and gram negative bacteria
Périodique
Journal of Inflammation
Auteur(s)
Lengacher  S., Jongeneel  C. V., Le Roy  D., Lee  J. D., Kravchenko  V., Ulevitch  R. J., Glauser  M. P., Heumann  D.
ISSN
1078-7852 (Print)
Statut éditorial
Publié
Date de publication
1995
Volume
47
Numéro
4
Pages
165-72
Notes
Comparative Study
In Vitro
Journal Article
Résumé
The serum lipopolysaccharide (LPS) binding protein, LBP, has been shown to greatly enhance cellular responses to low concentrations of LPS. Purified LBP facilitates the transfer of LPS to membrane-bound or soluble CD14; the CD14/LPS complex then triggers a signal in responsive cells. We have cloned and sequenced a cDNA encoding murine LBP, and produced recombinant murine LBP using a baculovirus expression system. Using either a solid-phase or a cytofluorometric assay, recombinant murine and human LBP were found to bind avidly to free LPS, but only weakly to live bacteria from most LPS-containing Gram negative strains. Binding correlated loosely with the length and composition of the polysaccharide O chains. However, recombinant LBP did bind well to all heat-killed bacterial preparations. These findings suggest that LBP could be implicated in the response to killed but not live Gram negative bacteria.
Mots-clé
*Acute-Phase Proteins Amino Acid Sequence Animals Baculoviridae/genetics Base Sequence Carrier Proteins/genetics/*metabolism Cell Line Cloning, Molecular DNA, Bacterial/genetics DNA, Complementary/genetics Escherichia coli/metabolism Gene Expression Gram-Negative Bacteria/*metabolism Heat Humans Lipopolysaccharides/chemistry/*metabolism *Membrane Glycoproteins Mice Molecular Sequence Data Recombinant Proteins/genetics/metabolism Sequence Homology, Amino Acid Spodoptera
Pubmed
Web of science
Création de la notice
24/01/2008 16:39
Dernière modification de la notice
20/08/2019 13:35
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