Tyrosine phosphorylation is an early and specific event involved in primary keratinocyte differentiation.

Détails

ID Serval
serval:BIB_0E5E909215FE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Tyrosine phosphorylation is an early and specific event involved in primary keratinocyte differentiation.
Périodique
Molecular and Cellular Biology
Auteur(s)
Filvaroff E., Stern D.F., Dotto G.P.
ISSN
0270-7306 (Print)
ISSN-L
0270-7306
Statut éditorial
Publié
Date de publication
1990
Volume
10
Numéro
3
Pages
1164-1173
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Very little is known about early molecular events triggering epithelial cell differentiation. We have examined the possible role of tyrosine phosphorylation in this process, as observed in cultures of primary mouse keratinocytes after exposure to calcium or 12-O-tetradecanoylphorbol-13-acetate (TPA). Immunoblotting with phosphotyrosine-specific antibodies as well as direct phosphoamino acid analysis revealed that induction of tyrosine phosphorylation occurs as a very early and specific event in keratinocyte differentiation. Very little or no induction of tyrosine phosphorylation was observed in a keratinocyte cell line resistant to the differentiating effects of calcium. Treatment of cells with tyrosine kinase inhibitors prevented induction of tyrosine phosphorylation by calcium and TPA and interfered with the differentiative effects of these agents. These results suggest that specific activation of tyrosine kinase(s) may play an important regulatory role in keratinocyte differentiation.
Mots-clé
Animals, Blotting, Western, Calcium/pharmacology, Cell Differentiation/drug effects, Cytoskeleton/metabolism, Cytosol/metabolism, Genistein, Isoflavones/pharmacology, Keratinocytes/cytology, Mice, Mice, Inbred Strains, Phosphoproteins/metabolism, Phosphotyrosine, Protein-Tyrosine Kinases/antagonists & inhibitors, Tetradecanoylphorbol Acetate/pharmacology, Transglutaminases/metabolism, Tyrosine/analogs & derivatives, Tyrosine/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 15:58
Dernière modification de la notice
20/08/2019 13:35
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