Functional Characterization of a Miniature Inverted Transposable Element at the Origin of mcr-5 Gene Acquisition in Escherichia coli.

Détails

ID Serval
serval:BIB_0E59C83B17BD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Functional Characterization of a Miniature Inverted Transposable Element at the Origin of mcr-5 Gene Acquisition in Escherichia coli.
Périodique
Antimicrobial agents and chemotherapy
Auteur(s)
Kieffer N., Nordmann P., Millemann Y., Poirel L.
ISSN
1098-6596 (Electronic)
ISSN-L
0066-4804
Statut éditorial
Publié
Date de publication
07/2019
Peer-reviewed
Oui
Volume
63
Numéro
7
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
Plasmid-mediated colistin resistance of the mobile colistin resistance (MCR) type is a growing concern in Enterobacteriaceae since it has been described worldwide in humans and animals. Here, we identified a series of MCR-producing Escherichia coli isolates corresponding to two different clones (represented by isolates PS1 and PS8b) producing MCR-1 and MCR-5, respectively, obtained from pig fecal samples in France. Plasmid analysis showed that the plasmid carrying the mcr-1 gene (pPS1) possesses an IncHI2 backbone, whereas the mcr-5 gene was carried onto a 6,268-bp nontypeable non-self-conjugative plasmid (pPS8b). Detailed analysis of plasmid pPS8b revealed a 3,803-bp-long cassette containing the mcr-5 gene that was bracketed by two inverted-repeat (IR) sequences with 5-bp-long direct repeats at each extremity, similarly to an insertion sequence, but with the exception that no transposase gene was identified within this cassette. By performing in vitro transposition experiments, we showed that the mcr-5 cassette could be mobilized by the TnAs1 transposase provided in trans, displaying a mobilization mechanism similar to that of miniature inverted-repeat transposable elements (MITEs).
Mots-clé
Animals, Anti-Bacterial Agents/pharmacology, Colistin/pharmacology, DNA Transposable Elements/genetics, Drug Resistance, Bacterial/genetics, Escherichia coli/drug effects, Escherichia coli/genetics, Escherichia coli Infections/drug therapy, Escherichia coli Infections/microbiology, Escherichia coli Proteins/genetics, France, Microbial Sensitivity Tests/methods, Plasmids/genetics, Swine, Transferases (Other Substituted Phosphate Groups)/genetics, MCR, MITE, colistin, mobilization, pig, resistance, transposition
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/06/2019 7:20
Dernière modification de la notice
20/06/2020 5:18
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