Spectrin subtypes in mammalian brain: an immunoelectron microscopic study.

Détails

ID Serval
serval:BIB_0E3D1D7CD8B0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Spectrin subtypes in mammalian brain: an immunoelectron microscopic study.
Périodique
Journal of Neuroscience
Auteur(s)
Zagon I.S., Higbee R., Riederer B.M., Goodman S.R.
ISSN
0270-6474 (Print)
ISSN-L
0270-6474
Statut éditorial
Publié
Date de publication
1986
Volume
6
Numéro
10
Pages
2977-2986
Langue
anglais
Résumé
Spectrin is a major cytoskeletal component of the brain. At least 2 distinct spectrin subtypes are found in mammalian brain: brain spectrin(240/235) and brain spectrin(240/235E). In the present study spectrin subtypes were localized in the adult mouse brain by immunoelectron microscopy using antibodies that recognize each subtype. Brain spectrin(240/235E) was concentrated in neuronal cell bodies, dendrites, and postsynaptic terminals. It was also prominently associated with the plasma membrane, microtubules, filaments, mitochondria, endoplasmic reticulum, and nuclear envelope, and it appeared to interconnect structural elements within the cell. Brain spectrin(240/235E) also was localized to the plasma membrane, nuclear envelope, and cytoplasmic organelles of glial cell bodies. Brain spectrin(240/235) was detected in axons and presynaptic elements, where it was associated with the plasma membrane, microtubules, filaments, synaptic vesicles, and mitochondria. These results show that spectrin is distributed throughout the cytoplasm of neural cells, the location of spectrin is dependent on subtype, and the cytoplasmic surface of plasma membrane and organelles contains an extensive and intricate spectrin meshwork.
Mots-clé
Animals, Antigens/analysis, Brain/metabolism, Brain/ultrastructure, Erythrocytes/metabolism, Immune Sera/immunology, Immunologic Techniques, Male, Mice, Mice, Inbred C57BL, Microscopy, Electron, Spectrin/blood, Spectrin/classification
Pubmed
Web of science
Création de la notice
24/01/2008 14:34
Dernière modification de la notice
20/08/2019 12:35
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