In vivo gene electroinjection and expression in rat liver
Détails
ID Serval
serval:BIB_0E33CC29A8BC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vivo gene electroinjection and expression in rat liver
Périodique
FEBS Letters
ISSN
0014-5793 (Print)
Statut éditorial
Publié
Date de publication
07/1996
Volume
389
Numéro
3
Pages
225-8
Notes
Journal Article --- Old month value: Jul 8
Résumé
In vivo targeted gene transfer by non-viral vectors is subjected to anatomical constraints depending on the route of administration. Transfection efficiency and gene expression in vivo using non-viral vectors is also relatively low. We report that in vivo electropermeabilization of the liver tissue of rats in the presence of genes encoding luciferase or beta-galactosidase resulted in the strong expression of these genetic markers in rat liver cells. About 30-40% of the rat liver cells electroporated expressed the beta-galactosidase genetic marker 48 h after electroporation. The marker expression was also detected at least 21 days after transfection at about 5% of the level 48 h after electroporation. The results indicate that gene transfer by electroporation in vivo may avoid anatomical constraints and low transfection efficiency.
Mots-clé
Animals
Electroporation/*methods
Flow Cytometry
*Gene Expression
*Gene Transfer Techniques
Liver/*metabolism
Luciferases/genetics/metabolism
Male
Plasmids
Rats
Rats, Sprague-Dawley
beta-Galactosidase/genetics/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 17:05
Dernière modification de la notice
20/08/2019 13:35