Delayed increase of extracellular arginine, the nitric oxide precursor, following electrical white matter stimulation in rat cerebellar slices.

Détails

ID Serval
serval:BIB_0DDFE55B6B96
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Delayed increase of extracellular arginine, the nitric oxide precursor, following electrical white matter stimulation in rat cerebellar slices.
Périodique
Neuroscience Letters
Auteur(s)
Hansel C., Batchelor A., Cuénod M., Garthwaite J., Knöpfel T., Do K.Q.
ISSN
0304-3940 (Print)
ISSN-L
0304-3940
Statut éditorial
Publié
Date de publication
1992
Peer-reviewed
Oui
Volume
142
Numéro
2
Pages
211-214
Langue
anglais
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Amino acid levels were measured in perfusates from biplanar slices of rat cerebellum installed in a Krebs-filled three-compartment chamber. The two lateral compartments housed the white matter and a section containing parallel fibres respectively. The central compartment housed cortical structures, including the Purkinje cell and granule cell bodies. This arrangement allows selective electrical stimulation of the parallel fibre or mossy fibre pathways, recording of the evoked responses to such stimulation and collection of the perfusion medium passing through the central chamber for amino acid analysis using high-pressure liquid chromatography (HPLC). Both, 2-Hz and 5-Hz stimulation of white matter caused a delayed increase in arginine levels in the perfusate. Since L-arginine is the physiological precursor of nitric oxide, a neuronal messenger in the brain, the data suggest that physiological stimuli can result in the release of this precursor, possibly to supply the nitric oxide synthase.
Mots-clé
Afferent Pathways/cytology, Afferent Pathways/metabolism, Animals, Arginine/metabolism, Cerebellum/metabolism, Cerebellum/physiology, Chromatography, High Pressure Liquid, Electric Stimulation, Female, Male, Nitric Oxide/metabolism, Purkinje Cells/physiology, Rats, Rats, Sprague-Dawley
Pubmed
Web of science
Création de la notice
06/03/2014 17:44
Dernière modification de la notice
20/08/2019 13:34
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