Imaging liver and brain glycogen metabolism at the nanometer scale.

Détails

ID Serval
serval:BIB_0D806AC07C94
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Imaging liver and brain glycogen metabolism at the nanometer scale.
Périodique
Nanomedicine
Auteur(s)
Takado Y., Knott G., Humbel B.M., Escrig S., Masoodi M., Meibom A., Comment A.
ISSN
1549-9642 (Electronic)
ISSN-L
1549-9634
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
11
Numéro
1
Pages
239-245
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
In mammals, glycogen synthesis and degradation are dynamic processes regulating blood and cerebral glucose-levels within a well-defined physiological range. Despite the essential role of glycogen in hepatic and cerebral metabolism, its spatiotemporal distribution at the molecular and cellular level is unclear. By correlating electron microscopy and ultra-high resolution ion microprobe (NanoSIMS) imaging of tissue from fasted mice injected with (13)C-labeled glucose, we demonstrate that liver glycogenesis initiates in the hepatocyte perinuclear region before spreading toward the cell membrane. In the mouse brain, we observe that (13)C is inhomogeneously incorporated into astrocytic glycogen at a rate ~25 times slower than in the liver, in agreement with prior bulk studies. This experiment, using temporally resolved, nanometer-scale imaging of glycogen synthesis and degradation, provides greater insight into glucose metabolism in mammalian organs and shows how this technique can be used to explore biochemical pathways in healthy and diseased states.
FROM THE CLINICAL EDITOR: By correlating electron microscopy and ultra-high resolution ion microprobe imaging of tissue from fasting mice injected with (13)C-labeled glucose, the authors demonstrate a method to image glycogen metabolism at the nanometer scale.
Pubmed
Web of science
Création de la notice
13/02/2015 17:20
Dernière modification de la notice
20/08/2019 12:34
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