Human polymeric IgA is superior to IgG and single-chain Fv of the same monoclonal specificity to inhibit urease activity associated with Helicobacter pylori.

Détails

ID Serval
serval:BIB_0D1D34FC6FAE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Human polymeric IgA is superior to IgG and single-chain Fv of the same monoclonal specificity to inhibit urease activity associated with Helicobacter pylori.
Périodique
Molecular Immunology
Auteur⸱e⸱s
Berdoz J., Corthésy B.
ISSN
0161-5890[print], 0161-5890[linking]
Statut éditorial
Publié
Date de publication
2004
Volume
41
Numéro
10
Pages
1013-1022
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Helicobacter-induced gastritis is considered nowadays an epidemic, the prevalence of which is one of the highest world-wide (70%), with as much as 40% of the population in industrialized countries. Helicobacter pylori (H. pylori) antigens (Ag) capable to elicit a protective immune response in animal models have been identified, but these antigens have not been shown to be strongly immunogenic when administered to humans. Due to their stability in the gastric environment and avidity, passive administration of secretory immunoglobulin A (SIgA) antibodies (Ab) targeting protective Ag might be particularly relevant as a substitute or complement to current therapies. To this aim, we have designed expression vectors to convert a scFv polypeptide specific for H. pylori urease subunit A into human IgG, polymeric IgA (IgAp/d) and SIgA. Purified proteins show proper binding characteristics toward both the native and denatured forms of H. pylori urease. The direct comparison between different isotype and molecular forms, but of unique specificity, demonstrates that SIgA and IgAp/d are more efficient in blocking free and H. pylori-associated urease than IgG and scFv. We conclude that the expression system reported herein will represent a valuable tool to produce human SIgA Ab of multiple specificities against H. pylori antigens involved in colonization and persistence.
Mots-clé
Animals, CHO Cells, Cloning, Molecular, Cricetinae, Helicobacter pylori/enzymology, Helicobacter pylori/immunology, Humans, Immunoglobulin A/genetics, Immunoglobulin A/immunology, Immunoglobulin G/genetics, Immunoglobulin G/immunology, Immunoglobulin Variable Region/genetics, Immunoglobulin Variable Region/immunology, Time Factors, Urease/antagonists &amp, inhibitors, Urease/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 14:53
Dernière modification de la notice
20/08/2019 12:34
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