Role of atrial natriuretic peptide in the suppression of lysophosphatydic acid-induced rat aortic smooth muscle (RASM) cell growth.
Détails
ID Serval
serval:BIB_0C8A3003877D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Role of atrial natriuretic peptide in the suppression of lysophosphatydic acid-induced rat aortic smooth muscle (RASM) cell growth.
Périodique
Molecular and cellular biochemistry
ISSN
0300-8177 (Print)
ISSN-L
0300-8177
Statut éditorial
Publié
Date de publication
04/2005
Volume
272
Numéro
1-2
Pages
19-28
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Lysophosphatidic acid (LPA) is a lipid mediator with multiple biological functions. In the present study we investigated the possible role of atrial natriuretic peptide (ANP), a hormone affecting cardiovascular homeostasis and inducing antimitogenic effects in different cell types, on LPA-induced cell growth and reactive oxygen species (ROS) production in rat aortic smooth muscle (RASM) cells. Both LPA effects on cell growth and levels of ROS were totally abrogated by physiological concentrations of ANP, without modifying the overexpression of LPA-receptors. These effects were also affected by cell pretreatment with wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K). Moreover, the LPA-induced activation of Akt, a downstream target of PI3K, was completely inhibited by physiological concentrations of ANP, which were also able to inhibit p42/p44 phosphorylation. Taken together, our data suggest that PI3K may represent an important step in the LPA signal transduction pathway responsible for ROS generation and DNA synthesis in RASM cells. At same time, the enzyme could also represent an essential target for the antiproliferative effects of ANP.
Mots-clé
Androstadienes/pharmacology, Animals, Aorta/cytology, Atrial Natriuretic Factor/pharmacology, Atrial Natriuretic Factor/physiology, Cells, Cultured, DNA Replication/drug effects, Enzyme Activation, Lysophospholipids/antagonists & inhibitors, Lysophospholipids/pharmacology, Male, Mitogen-Activated Protein Kinase 1/metabolism, Mitogen-Activated Protein Kinase 3/metabolism, Muscle, Smooth, Vascular/cytology, Muscle, Smooth, Vascular/drug effects, Muscle, Smooth, Vascular/metabolism, Phosphatidylinositol 3-Kinases/antagonists & inhibitors, Phosphorylation, Protein Kinase Inhibitors/pharmacology, Protein-Serine-Threonine Kinases/metabolism, Proto-Oncogene Proteins/agonists, Proto-Oncogene Proteins/metabolism, Proto-Oncogene Proteins c-akt, Rats, Rats, Wistar, Reactive Oxygen Species/metabolism, Receptors, Lysophosphatidic Acid/genetics, Receptors, Lysophosphatidic Acid/metabolism, Signal Transduction
Pubmed
Création de la notice
06/03/2017 17:23
Dernière modification de la notice
20/08/2019 12:34