Expression of pSTAT5 predicts FLT3 internal tandem duplications in acute myeloid leukemia.

Détails

ID Serval
serval:BIB_0C70C2A6D0DA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Expression of pSTAT5 predicts FLT3 internal tandem duplications in acute myeloid leukemia.
Périodique
Annals of Hematology
Auteur(s)
Obermann Ellen Christina (co-premier), Arber Caroline (co-premier), Jotterand Martine, Tichelli Andre, Hirschmann Petra, Tzankov Alexandar
Statut éditorial
Publié
Date de publication
2010
Volume
89
Numéro
7
Pages
663-669
Langue
anglais
Résumé
Mutations of the Fms-like tyrosine kinase 3 (FLT3) can be detected in a significant number of acute myeloid leukemias (AML). Seventy-five cases of acute myeloid leukemia were evaluated for FLT3-internal tandem duplications (ITD) by polymerase chain reaction. Paraffin-embedded formalin-fixed trephine biopsies of these cases were evaluated for expression of phosphorylated signal transducer and activator of transcription 1 (pSTAT1), pSTAT3, and pSTAT5. Specific expression of pSTAT5 was proven in leukemic blasts in situ by double staining with a blast-specific marker. Expression of pSTAT5 in > or =1% of blasts was highly predictive of FLT3-ITD. Neither expression of pSTAT1 nor pSTAT3 were associated with FLT3 mutations. Altogether we conclude that pSTAT5 expression can precisely be assessed by immunohistochemistry in routinely processed bone marrow trephines, STAT5 is highly likely the preferred second messenger of FLT3-mediated signaling in AML, and expression of pSTAT5 is predictive of FLT3-ITD.
Mots-clé
Adult, Aged, Aged, 80 and over, Blast Crisis/genetics, Blast Crisis/metabolism, Female, Gene Expression Regulation, Leukemic, Humans, Leukemia, Myeloid, Acute/genetics, Leukemia, Myeloid, Acute/metabolism, Male, Middle Aged, Mutation, Phosphorylation/genetics, Predictive Value of Tests, Retrospective Studies, STAT1 Transcription Factor/genetics, STAT1 Transcription Factor/metabolism, STAT3 Transcription Factor/genetics, STAT3 Transcription Factor/metabolism, STAT5 Transcription Factor/biosynthesis, STAT5 Transcription Factor/genetics, Second Messenger Systems, fms-Like Tyrosine Kinase 3/genetics, fms-Like Tyrosine Kinase 3/metabolism
Pubmed
Web of science
Création de la notice
08/06/2010 16:47
Dernière modification de la notice
01/11/2019 11:20
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