Translocation of the yeast dolichol-phosphate-mannose synthase into microsomal membranes.

Détails

ID Serval
serval:BIB_0C3D172F7AE0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Translocation of the yeast dolichol-phosphate-mannose synthase into microsomal membranes.
Périodique
Biochemical and Biophysical Research Communications
Auteur(s)
Déglon N., Krapp A., Bron C., Fasel N.
ISSN
0006-291X (Print)
ISSN-L
0006-291X
Statut éditorial
Publié
Date de publication
1991
Volume
174
Numéro
3
Pages
1337-1342
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Dolichol-phosphate-mannose synthase catalyzes the formation of Dolichol-phosphate-mannose from Dolichol-phosphate and GDP-mannose. Analysis of the primary amino acid sequence of the yeast enzyme predicts a luminal orientation of the enzyme in the endoplasmic reticulum. We analysed the translocation of the Dolichol-phosphate-mannose synthase into dog pancreatic microsomal membranes: resistance to proteolytic attack provides evidence of its luminal orientation and asks for a reevaluation of the topology of the reaction.
Mots-clé
Animals, Base Sequence, DNA, Fungal/genetics, DNA, Fungal/isolation & purification, Dogs, Genes, Fungal, Intracellular Membranes/enzymology, Mannosyltransferases/genetics, Mannosyltransferases/isolation & purification, Microsomes/enzymology, Molecular Sequence Data, Oligonucleotide Probes, Pancreas/enzymology, Polymerase Chain Reaction, Protein Biosynthesis, RNA, Messenger/genetics, RNA, Messenger/metabolism, Rabbits, Restriction Mapping, Reticulocytes/metabolism, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae/genetics, Transcription, Genetic
Pubmed
Web of science
Création de la notice
24/01/2008 15:02
Dernière modification de la notice
20/08/2019 12:33
Données d'usage