Mast cells are critical mediators of vaccine-induced Helicobacter clearance in the mouse model

Détails

ID Serval
serval:BIB_0C18B5EDBB1A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Mast cells are critical mediators of vaccine-induced Helicobacter clearance in the mouse model
Périodique
Gastroenterology
Auteur(s)
Velin  D., Bachmann  D., Bouzourene  H., Michetti  P.
ISSN
0016-5085 (Print)
Statut éditorial
Publié
Date de publication
07/2005
Volume
129
Numéro
1
Pages
142-55
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
BACKGROUND & AIMS: Despite the proven ability of immunization to prevent Helicobacter infection in mouse models, the precise mechanism of protection has remained elusive. METHODS: We explored the cellular events associated with Helicobacter clearance from the stomach following vaccination by flow cytometry analysis and histological and molecular studies. RESULTS: Kinetic studies showed that the infection is undetectable in vaccinated mice at day 5 postbacterial challenge. Flow cytometry analysis showed that the percentages of mast cells (CD3 - CD117 + ) increased in the lymphoid cells isolated from the stomach at day 4 postchallenge in urease + cholera toxin (CT)-vaccinated mice in comparison with mice administered with CT alone (9.4% +/- 4.4% and 3.1% +/- 1%, respectively, for vaccinated and CT administered, n = 5; P < .01). Quantitative PCR analysis showed an increased messenger RNA (mRNA) expression of the mast cell proteases 1 and 2 at day 5 postchallenge in the stomach of vaccinated mice. In contrast to wild-type mice, mast cell-deficient mice (W/W v mice) were not protected from H felis colonization after vaccination. Indeed only 1 out of 12 vaccinated W/W v mice showed a negative urease test. Remarkably, vaccinated W/W v mice reconstituted with cultured bone marrow-derived mast cells recovered the ability to clear the infection after vaccination (8 out of 10 mast cell-reconstituted mice showed negative urease tests [ P < .006 as compared with wild-type mice]). CONCLUSIONS: These experiments show that mast cells are, unexpectedly, critical mediators of anti- Helicobacter vaccination.
Mots-clé
Administration, Intranasal Animals Bacterial Vaccines/*pharmacology Bone Marrow Cells/immunology/microbiology CD4-Positive T-Lymphocytes/immunology/microbiology Chymases Disease Models, Animal Female Gastric Mucosa/immunology/microbiology Helicobacter Infections/*immunology/*prevention & control Helicobacter felis/immunology Helicobacter pylori/*immunology Immunoglobulin E/immunology Male Mast Cells/*immunology/microbiology Mice Mice, Inbred C57BL Mice, Mutant Strains Neutrophils/immunology Proto-Oncogene Proteins c-kit/genetics RNA, Messenger/analysis Serine Endopeptidases/blood/genetics Urease/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 16:10
Dernière modification de la notice
20/08/2019 12:33
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