Efficient T cell repertoire selection in tetraparental chimeric mice independent of thymic epithelial MHC
Détails
ID Serval
serval:BIB_0C0DA4D9366A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Efficient T cell repertoire selection in tetraparental chimeric mice independent of thymic epithelial MHC
Périodique
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
02/2003
Volume
100
Numéro
4
Pages
1861-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb 18
Research Support, Non-U.S. Gov't --- Old month value: Feb 18
Résumé
Nonthymic epithelial cells were compared with thymic epithelial cells for their role in T cell repertoire selection. Tetraparental aggregation chimeras were generated from T and B cell-deficient mice (H-2(d) SCID or H-2(b) Rag-/-) and thymus-deficient nude mice (H-2(b) or H-2(d)). These tetraparental mice showed primary protective CD8(+) T cell responses, after lymphocytic choriomeningitis virus infection, that were peptide-specifically restricted to either thymic or nonthymic epithelial MHC at comparable levels. These chimeras also mounted neutralizing IgG responses dependent on cognate CD4(+) T helper cell activity restricted to nonthymic epithelial MHC. Therefore, in contrast to earlier results with irradiation or thymus chimeras, these relatively undisturbed tetraparental mice reveal that the MHC of nonthymic epithelial cells efficiently selects a functional T cell repertoire.
Mots-clé
Animals
CD4-Positive T-Lymphocytes/*immunology
Cell Line
*Chimera
Enzyme-Linked Immunosorbent Assay
Epithelial Cells/immunology
Flow Cytometry
*Major Histocompatibility Complex
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, SCID
Microscopy, Fluorescence
Thymus Gland/*cytology/immunology
Pubmed
Web of science
Création de la notice
28/01/2008 12:32
Dernière modification de la notice
20/08/2019 13:33