Novel Approaches for Fungal Transcriptomics from Host Samples.

Détails

Ressource 1Télécharger: 26834721_BIB_0BC83D13D4FD.pdf (1943.22 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_0BC83D13D4FD
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Novel Approaches for Fungal Transcriptomics from Host Samples.
Périodique
Frontiers In Microbiology
Auteur⸱e⸱s
Amorim-Vaz S., Sanglard D.
ISSN
1664-302X (Electronic)
ISSN-L
1664-302X
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
6
Pages
1571
Langue
anglais
Notes
Publication types: Journal Article ; ReviewPublication Status: epublishDocument Type: Review
Résumé
Candida albicans adaptation to the host requires a profound reprogramming of the fungal transcriptome as compared to in vitro laboratory conditions. A detailed knowledge of the C. albicans transcriptome during the infection process is necessary in order to understand which of the fungal genes are important for host adaptation. Such genes could be thought of as potential targets for antifungal therapy. The acquisition of the C. albicans transcriptome is, however, technically challenging due to the low proportion of fungal RNA in host tissues. Two emerging technologies were used recently to circumvent this problem. One consists of the detection of low abundance fungal RNA using capture and reporter gene probes which is followed by emission and quantification of resulting fluorescent signals (nanoString). The other is based first on the capture of fungal RNA by short biotinylated oligonucleotide baits covering the C. albicans ORFome permitting fungal RNA purification. Next, the enriched fungal RNA is amplified and subjected to RNA sequencing (RNA-seq). Here we detail these two transcriptome approaches and discuss their advantages and limitations and future perspectives in microbial transcriptomics from host material.
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/02/2016 17:37
Dernière modification de la notice
20/08/2019 12:33
Données d'usage