Functional analysis of the signal-sequence processing site of yeast acid phosphatase
Détails
ID Serval
serval:BIB_0AC7003ECC83
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Functional analysis of the signal-sequence processing site of yeast acid phosphatase
Périodique
European Journal of Biochemistry
ISSN
0014-2956 (Print)
Statut éditorial
Publié
Date de publication
06/1989
Volume
182
Numéro
2
Pages
213-21
Notes
Journal Article --- Old month value: Jun 15
Résumé
A systematic study of the signal peptidase cleavage site of the main cell-wall-repressible Saccharomyces cerevisiae acid phosphatase encoded by the PHO5 gene is presented. The last amino acid of the signal sequence, the chromosomally encoded alanine of the wild-type gene, was changed by any of 19 other amino acids in the chromosomal DNA by using in vitro mutagenesis in Escherichia coli and the technique of gene replacement. Processing and secretion are normal when the amino acid at this position is a small neutral amino acid, i.e. alanine, glycine, cysteine, serine or threonine. Processing glycosylation, and secretion of regulated acid phosphatase are distinctly affected with other amino acid substitutions and core-glycosylated protein accumulates in the cell. Surprisingly, PHO5 protein is still secreted to the cell wall and into the growth medium but at a lower rate and without cleavage of the signal sequence. The same features are exhibited by a mutated acid phosphatase with a deletion of four amino acids at the end of the signal peptide (-7 to -4 relative to the processing site) thus preserving the important -3 to -1 region.
Mots-clé
Acetylglucosaminidase
Acid Phosphatase/*genetics
Alanine/analysis
Amino Acid Sequence
Base Sequence
Cell Wall/enzymology
Electrophoresis, Polyacrylamide Gel
Endopeptidases/metabolism
Escherichia coli/genetics
*Genes
*Genes, Fungal
*Genetic Vectors
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
*Membrane Proteins
Molecular Sequence Data
Mutation
Plasmids
Protein Sorting Signals/*analysis
Saccharomyces cerevisiae/*enzymology/genetics
Sequence Homology, Nucleic Acid
*Serine Endopeptidases
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 17:47
Dernière modification de la notice
20/08/2019 13:32