Method for in situ hybridization to polytene chromosomes from ovarian nurse cells of Anopheles gambiae (Diptera: Culicidae)
Détails
ID Serval
serval:BIB_0AA8D3976FCC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Method for in situ hybridization to polytene chromosomes from ovarian nurse cells of Anopheles gambiae (Diptera: Culicidae)
Périodique
Journal of Medical Entomology
ISSN
0022-2585 (Print)
Statut éditorial
Publié
Date de publication
09/1990
Volume
27
Numéro
5
Pages
905-12
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
A procedure for in situ hybridization to polytene chromosomes from the ovarian nurse cells of Anopheles gambiae Giles has been developed. This procedure involves a modification of established methods for Drosophila larval salivary gland polytene chromosomes. Treatment of chromosome squashes with xylene followed by slow rehydration provides required resolution of chromosome banding patterns, possibly because fatty contaminants are removed from ovarian nurse cell preparations. Using this procedure, unique DNA sequences from a genomic library of An. gambiae have been mapped on adult mosquito polytene chromosomes. The ability to locate genetic markers on chromosomes will allow correlation of physical and genetic maps. Such maps will facilitate identification of genetic loci and expand our knowledge of the genomic organization of An. gambiae.
Mots-clé
Animals
Anopheles/*genetics
Blotting, Southern
Chromosome Banding
Chromosome Mapping
DNA/*analysis
Female
Nucleic Acid Hybridization
Pubmed
Web of science
Création de la notice
25/01/2008 15:58
Dernière modification de la notice
20/08/2019 13:32