Modulation of drought resistance by the abscisic acid receptor PYL5 through inhibition of clade A PP2Cs.

Détails

ID Serval
serval:BIB_0A5BFB69C336
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Modulation of drought resistance by the abscisic acid receptor PYL5 through inhibition of clade A PP2Cs.
Périodique
The Plant journal
Auteur(s)
Santiago J., Rodrigues A., Saez A., Rubio S., Antoni R., Dupeux F., Park S.Y., Márquez J.A., Cutler S.R., Rodriguez P.L.
ISSN
1365-313X (Electronic)
ISSN-L
0960-7412
Statut éditorial
Publié
Date de publication
11/2009
Peer-reviewed
Oui
Volume
60
Numéro
4
Pages
575-588
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Abscisic acid (ABA) is a key phytohormone involved in adaption to environmental stress and regulation of plant development. Clade A protein phosphatases type 2C (PP2Cs), such as HAB1, are key negative regulators of ABA signaling in Arabidopsis. To obtain further insight into regulation of HAB1 function by ABA, we have screened for HAB1-interacting partners using a yeast two-hybrid approach. Three proteins were identified, PYL5, PYL6 and PYL8, which belong to a 14-member subfamily of the Bet v1-like superfamily. HAB1-PYL5 interaction was confirmed using BiFC and co-immunoprecipitation assays. PYL5 over-expression led to a globally enhanced response to ABA, in contrast to the opposite phenotype reported for HAB1-over-expressing plants. F(2) plants that over-expressed both HAB1 and PYL5 showed an enhanced response to ABA, indicating that PYL5 antagonizes HAB1 function. PYL5 and other members of its protein family inhibited HAB1, ABI1 and ABI2 phosphatase activity in an ABA-dependent manner. Isothermal titration calorimetry revealed saturable binding of (+)ABA to PYL5, with K(d) values of 1.1 mum or 38 nm in the absence or presence of the PP2C catalytic core of HAB1, respectively. Our work indicates that PYL5 is a cytosolic and nuclear ABA receptor that activates ABA signaling through direct inhibition of clade A PP2Cs. Moreover, we show that enhanced resistance to drought can be obtained through PYL5-mediated inhibition of clade A PP2Cs.
Mots-clé
Abscisic Acid/pharmacology, Arabidopsis/enzymology, Arabidopsis/genetics, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Droughts, Gene Expression Regulation, Plant, Phosphoprotein Phosphatases/genetics, Phosphoprotein Phosphatases/metabolism, Protein Phosphatase 2C, RNA, Plant/genetics, Recombinant Proteins/genetics, Recombinant Proteins/metabolism, Signal Transduction
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/01/2019 18:25
Dernière modification de la notice
21/08/2019 6:35
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