Typing of Pneumocystis carinii f. sp. hominis by single-strand conformation polymorphism of four genomic regions

Détails

ID Serval
serval:BIB_0A34C0CA5DF7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Typing of Pneumocystis carinii f. sp. hominis by single-strand conformation polymorphism of four genomic regions
Périodique
Journal of Clinical Microbiology
Auteur(s)
Hauser  P. M., Francioli  P., Bille  J., Telenti  A., Blanc  D. S.
ISSN
0095-1137 (Print)
Statut éditorial
Publié
Date de publication
12/1997
Volume
35
Numéro
12
Pages
3086-91
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
To better investigate Pneumocystis carinii f. sp. hominis epidemiology, we have developed a molecular typing method. Because of the limited genetic variability of the P. carinii hominis genome, a multitarget approach was used. Four variable regions of the genome were amplified by PCR, polymorphism in each region was assessed by the single-strand conformation polymorphism (SSCP) technique, and the results for the four regions of each patient were combined. Bronchoalveolar lavage specimens collected from 11 patients were examined. Four patients were probably infected by a single strain, since their specimens yielded simple SSCP patterns (two bands corresponding to one allele). The combinations of these patterns were unique, suggesting that the strains which infected these patients were different. For the other seven patients, complex patterns were found (three or four bands corresponding to two alleles). The presence of more than one allele of a region in a patient is likely to be due to coinfection. Polymorphism was also assessed by sequencing, which revealed variations at nucleotide positions previously reported to vary. About half of the observed alleles had already been reported by laboratories in different countries. Multitarget typing of P. carinii hominis by PCR-SSCP should allow investigation of strain diversity and thus be useful for future epidemiological studies.
Mots-clé
AIDS-Related Opportunistic Infections/epidemiology/microbiology Alleles Base Sequence Bronchoalveolar Lavage Fluid/microbiology Cloning, Molecular DNA Primers/genetics Epidemiology, Molecular *Genome, Fungal Humans Pneumocystis/*classification/*genetics/isolation & purification Pneumonia, Pneumocystis/complications/epidemiology/microbiology Polymerase Chain Reaction *Polymorphism, Single-Stranded Conformational Switzerland/epidemiology Variation (Genetics)
Pubmed
Web of science
Création de la notice
25/01/2008 18:08
Dernière modification de la notice
20/08/2019 13:32
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