Which prior knowledge? Quantification of in vivo brain 13C MR spectra following 13C glucose infusion using AMARES.

Détails

ID Serval
serval:BIB_09BA25ACE89A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Which prior knowledge? Quantification of in vivo brain 13C MR spectra following 13C glucose infusion using AMARES.
Périodique
Magnetic Resonance in Medicine
Auteur(s)
Lanz B., Duarte J.M., Kunz N., Mlynárik V., Gruetter R., Cudalbu C.
ISSN
1522-2594 (Electronic)
ISSN-L
0740-3194
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
69
Numéro
6
Pages
1512-1522
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
The recent developments in high magnetic field 13C magnetic resonance spectroscopy with improved localization and shimming techniques have led to important gains in sensitivity and spectral resolution of 13C in vivo spectra in the rodent brain, enabling the separation of several 13C isotopomers of glutamate and glutamine. In this context, the assumptions used in spectral quantification might have a significant impact on the determination of the 13C concentrations and the related metabolic fluxes. In this study, the time domain spectral quantification algorithm AMARES (advanced method for accurate, robust and efficient spectral fitting) was applied to 13 C magnetic resonance spectroscopy spectra acquired in the rat brain at 9.4 T, following infusion of [1,6-(13)C2 ] glucose. Using both Monte Carlo simulations and in vivo data, the goal of this work was: (1) to validate the quantification of in vivo 13C isotopomers using AMARES; (2) to assess the impact of the prior knowledge on the quantification of in vivo 13C isotopomers using AMARES; (3) to compare AMARES and LCModel (linear combination of model spectra) for the quantification of in vivo 13C spectra. AMARES led to accurate and reliable 13C spectral quantification similar to those obtained using LCModel, when the frequency shifts, J-coupling constants and phase patterns of the different 13C isotopomers were included as prior knowledge in the analysis.
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/04/2013 11:52
Dernière modification de la notice
20/08/2019 12:31
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