Secreted metalloprotease gene family of Microsporum canis

Détails

ID Serval
serval:BIB_0983FF268CAA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Secreted metalloprotease gene family of Microsporum canis
Périodique
Infection and Immunity
Auteur(s)
Brouta  F., Descamps  F., Monod  M., Vermout  S., Losson  B., Mignon  B.
ISSN
0019-9567 (Print)
Statut éditorial
Publié
Date de publication
10/2002
Volume
70
Numéro
10
Pages
5676-83
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
Keratinolytic proteases secreted by dermatophytes are likely to be virulence-related factors. Microsporum canis, the main agent of dermatophytosis in dogs and cats, causes a zoonosis that is frequently reported. Using Aspergillus fumigatus metalloprotease genomic sequence (MEP) as a probe, three genes (MEP1, MEP2, and MEP3) were isolated from an M. canis genomic library. They presented a quite-high percentage of identity with both A. fumigatus MEP and Aspergillus oryzae neutral protease I genes. At the amino acid level, they all contained an HEXXH consensus sequence, confirming that these M. canis genes (MEP genes) encode a zinc-containing metalloprotease gene family. Furthermore, MEP3 was found to be the gene encoding a previously isolated M. canis 43.5-kDa keratinolytic metalloprotease, and was successfully expressed as an active recombinant enzyme in Pichia pastoris. Reverse transcriptase nested PCR performed on total RNA extracted from the hair of M. canis-infected guinea pigs showed that at least MEP2 and MEP3 are produced during the infection process. This is the first report describing the isolation of a gene family encoding potential virulence-related factors in dermatophytes.
Mots-clé
Amino Acid Sequence Animals Aspergillus fumigatus/enzymology/genetics Aspergillus oryzae/enzymology/genetics Base Sequence Cats Cloning, Molecular DNA, Fungal/genetics Dermatomycoses/etiology Dogs *Fungal Proteins Gene Expression *Genes, Fungal Humans Metalloendopeptidases/*genetics/secretion Microsporum/*enzymology/*genetics/pathogenicity Molecular Sequence Data *Multigene Family Pichia/genetics Recombinant Proteins/secretion Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Virulence/genetics/physiology Zoonoses/etiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:46
Dernière modification de la notice
20/08/2019 12:31
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