Photodynamic therapy enhances liposomal doxorubicin distribution in tumors during isolated perfusion of rodent lungs.
Détails
ID Serval
serval:BIB_0954EB6F374F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Photodynamic therapy enhances liposomal doxorubicin distribution in tumors during isolated perfusion of rodent lungs.
Périodique
European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
ISSN
1421-9921 (Electronic)
ISSN-L
0014-312X
Statut éditorial
Publié
Date de publication
2011
Volume
47
Numéro
4
Pages
196-204
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Résumé
BACKGROUND: Photodynamic therapy (PDT) at low drug-light conditions can enhance the transport of intravenously injected macromolecular therapeutics through the tumor vasculature. Here we determined the impact of PDT on the distribution of liposomal doxorubicin (Liporubicin™) administered by isolated lung perfusion (ILP) in sarcomas grown on rodent lungs.
METHODS: A syngeneic methylcholanthrene-induced sarcoma cell line was implanted subpleurally in the left lung of Fischer rats. Treatment schemes consisted in ILP alone (400 μg of Liporubicin), low-dose (0.0625 mg/kg Visudyne®, 10 J/cm(2) and 35 mW/cm(2)) and high-dose left lung PDT (0.125 mg/kg Visudyne, 10 J/cm(2) and 35 mW/cm(2)) followed by ILP (400 μg of Liporubicin). The uptake and distribution of Liporubicin in tumor and lung tissues were determined by high-performance liquid chromatography and fluorescence microscopy in each group.
RESULTS: Low-dose PDT significantly improved the distribution of Liporubicin in tumors compared to high-dose PDT (p < 0.05) and ILP alone (p < 0.05). However, both PDT pretreatments did not result in a higher overall drug uptake in tumors or a higher tumor-to-lung drug ratio compared to ILP alone.
CONCLUSIONS: Intraoperative low-dose Visudyne-mediated PDT enhances liposomal doxorubicin distribution administered by ILP in sarcomas grown on rodent lungs which is predicted to improve tumor control by ILP.
METHODS: A syngeneic methylcholanthrene-induced sarcoma cell line was implanted subpleurally in the left lung of Fischer rats. Treatment schemes consisted in ILP alone (400 μg of Liporubicin), low-dose (0.0625 mg/kg Visudyne®, 10 J/cm(2) and 35 mW/cm(2)) and high-dose left lung PDT (0.125 mg/kg Visudyne, 10 J/cm(2) and 35 mW/cm(2)) followed by ILP (400 μg of Liporubicin). The uptake and distribution of Liporubicin in tumor and lung tissues were determined by high-performance liquid chromatography and fluorescence microscopy in each group.
RESULTS: Low-dose PDT significantly improved the distribution of Liporubicin in tumors compared to high-dose PDT (p < 0.05) and ILP alone (p < 0.05). However, both PDT pretreatments did not result in a higher overall drug uptake in tumors or a higher tumor-to-lung drug ratio compared to ILP alone.
CONCLUSIONS: Intraoperative low-dose Visudyne-mediated PDT enhances liposomal doxorubicin distribution administered by ILP in sarcomas grown on rodent lungs which is predicted to improve tumor control by ILP.
Mots-clé
Animals, Antibiotics, Antineoplastic/analysis, Antibiotics, Antineoplastic/pharmacology, Cell Line, Tumor, Chromatography, High Pressure Liquid, Doxorubicin/analysis, Doxorubicin/pharmacology, Liposomes/administration & dosage, Lung/chemistry, Lung/drug effects, Lung Neoplasms/drug therapy, Lung Neoplasms/pathology, Male, Microscopy, Fluorescence, Perfusion, Photochemotherapy, Photosensitizing Agents/pharmacology, Photosensitizing Agents/therapeutic use, Porphyrins/pharmacology, Porphyrins/therapeutic use, Rats, Rats, Inbred F344, Sarcoma/drug therapy, Sarcoma/pathology
Pubmed
Web of science
Création de la notice
28/10/2011 11:01
Dernière modification de la notice
20/08/2019 13:31