Importance of sequences adjacent to the terminal tripeptide in the import of a peroxisomal Candida tropicalis protein in plant peroxisomes.

Détails

ID Serval
serval:BIB_08F046C2A2B7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Importance of sequences adjacent to the terminal tripeptide in the import of a peroxisomal Candida tropicalis protein in plant peroxisomes.
Périodique
Planta
Auteur(s)
Bongcam V., MacDonald-Comber Petétot J., Mittendorf V., Robertson E.J., Leech R.M., Qin Y.M., Hiltunen J.K., Poirier Y.
ISSN
0032-0935 (Print)
ISSN-L
0032-0935
Statut éditorial
Publié
Date de publication
2000
Volume
211
Numéro
1
Pages
150-157
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The peroxisome targeting signal (PTS) required for import of the rat acyl-CoA oxidase (AOX; EC 1.3.3.6) and the Candida tropicalis multifunctional protein (MFP) in plant peroxisomes was assessed in transgenic Arabidopsis thaliana (L.) Heynh. The native rat AOX accumulated in peroxisomes in A. thaliana cotyledons and targeting was dependent on the presence of the C-terminal tripeptide S-K-L. In contrast, the native C. tropicalis MFP, containing the consensus PTS sequence A-K-I was not targeted to plant peroxisomes. Modification of the carboxy terminus to the S-K-L tripeptide also failed to deliver the MFP to peroxisomes while addition of the last 34 amino acids of the Brassica napus isocitrate lyase, containing the terminal tripeptide S-R-M, enabled import of the fusion protein into peroxisomes. These results underline the influence of the amino acids adjacent to the terminal tripeptide of the C. tropicalis MFP on peroxisomal targeting, even in the context of a protein having a consensus PTS sequence S-K-L.
Mots-clé
Acyl-CoA Oxidase, Animals, Arabidopsis/metabolism, Arabidopsis Proteins, Candida/genetics, Candida/metabolism, Fungal Proteins/metabolism, Fungal Proteins/physiology, Oxidoreductases/metabolism, Peroxisomes/genetics, Peroxisomes/metabolism, Plants, Genetically Modified/metabolism, Rats, Receptors, Cytoplasmic and Nuclear/physiology
Pubmed
Web of science
Création de la notice
24/01/2008 20:43
Dernière modification de la notice
20/08/2019 13:31
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