Structure, genetic localization, and identification of the cardiac and skeletal muscle transcripts of the human integrin alpha7 gene (ITGA7)

Détails

ID Serval
serval:BIB_08A54AF26488
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Structure, genetic localization, and identification of the cardiac and skeletal muscle transcripts of the human integrin alpha7 gene (ITGA7)
Périodique
Biochemical and Biophysical Research Communications
Auteur⸱e⸱s
Vignier  N., Moghadaszadeh  B., Gary  F., Beckmann  J., Mayer  U., Guicheney  P.
ISSN
0006-291X (Print)
Statut éditorial
Publié
Date de publication
07/1999
Volume
260
Numéro
2
Pages
357-64
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 5
Résumé
We have determined the structure and the exon size pattern of the human integrin alpha7 subunit gene (ITGA7), which has been shown to be affected in a form of congenital myopathy. The gene is composed of at least 27 exons spanning a region of about 22.5 kb. The sequence of all exon/intron boundaries was determined and conforms to the GT/AG splicing consensus. We investigated the different splicing forms previously described in human and rodents. The major cytoplasmic variants alpha7A and alpha7B, which are developmentally regulated and tissue specific, were identified in human tissues, as well as the extracellular isoforms X1 and X2. The recently described D variant was detected in adult tissues by RT-PCR but not the C variant. We localized ITGA7 on chromosome 12q13 by high-resolution radiation hybrid mapping between D12S312 and D12S90 and identified a new CA-repeat microsatellite in intron 1.
Mots-clé
Amino Acid Sequence Animals Antigens, CD/*genetics Base Sequence Chromosome Mapping Chromosomes, Human, Pair 12 DNA, Complementary Dinucleotide Repeats Exons Humans *Integrin alpha Chains Introns Mice Molecular Sequence Data Muscle, Skeletal/*metabolism Myocardium/*metabolism RNA Splicing RNA, Messenger/*genetics/metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid
Pubmed
Web of science
Création de la notice
25/01/2008 16:17
Dernière modification de la notice
20/08/2019 12:30
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