Brain-derived neurotrophic factor enhances the hippocampal expression of key postsynaptic proteins in vivo including the monocarboxylate transporter MCT2.

Détails

ID Serval
serval:BIB_08361BDA1D3C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Brain-derived neurotrophic factor enhances the hippocampal expression of key postsynaptic proteins in vivo including the monocarboxylate transporter MCT2.
Périodique
Neuroscience
Auteur(s)
Robinet C., Pellerin L.
ISSN
1873-7544 (Electronic)
ISSN-L
0306-4522
Statut éditorial
Publié
Date de publication
2011
Volume
192
Pages
155-163
Langue
anglais
Résumé
Brain-derived neurotrophic factor (BDNF) promotes synaptic plasticity via an enhancement in expression of specific synaptic proteins. Recent results suggest that the neuronal monocarboxylate transporter MCT2 is a postsynaptic protein critically involved in synaptic plasticity and long-term memory. To investigate in vivo whether BDNF can modulate the expression of MCT2 as well as other proteins involved in synaptic plasticity, acute injection of BDNF was performed in mouse dorsal hippocampal CA1 area. Using immunohistochemistry, it was found that MCT2 expression was enhanced in part of the CA1 area and in the dentate gyrus 6 h after a single intrahippocampal injection of BDNF. Similarly, expression of the immediate early genes Arc and Zif268 was enhanced in the same hippocampal areas, in accordance with their role in synaptic plasticity. Immunoblot analysis confirmed the significant enhancement in MCT2 protein expression. In contrast, no changes were observed for the glial monocarboxylate transporters MCT1 and MCT4. When other synaptic proteins were investigated, it was found that postsynaptic density 95 (PSD95) and glutamate receptor 2 (GluR2) protein levels were significantly enhanced while no effect could be detected for synaptophysin, synaptosomal-associated protein 25 (SNAP25), αCaMKII and GluR1. These results demonstrate that MCT2 expression can be upregulated together with other key postsynaptic proteins in vivo under conditions related to synaptic plasticity, further suggesting the importance of energetics for memory formation.
Mots-clé
Animals, Blotting, Western, Brain-Derived Neurotrophic Factor/metabolism, Brain-Derived Neurotrophic Factor/pharmacology, Hippocampus/metabolism, Immunohistochemistry, Male, Mice, Mice, Inbred C57BL, Monocarboxylic Acid Transporters/metabolism, Neuronal Plasticity/physiology, Up-Regulation
Pubmed
Web of science
Création de la notice
03/11/2011 10:14
Dernière modification de la notice
20/08/2019 13:30
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