Anticlonotypic monoclonal antibodies induce proliferation of clonotype-positive T cells in peripheral blood human T lymphocytes. Evidence for a phenotypic (T4/T8) heterogeneity of the clonotype-positive proliferating cells

Détails

ID Serval
serval:BIB_080C78C5DEC4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Anticlonotypic monoclonal antibodies induce proliferation of clonotype-positive T cells in peripheral blood human T lymphocytes. Evidence for a phenotypic (T4/T8) heterogeneity of the clonotype-positive proliferating cells
Périodique
Journal of Experimental Medicine
Auteur⸱e⸱s
Moretta  A., Pantaleo  G., Lopez-Botet  M., Mingari  M. C., Moretta  L.
ISSN
0022-1007 (Print)
Statut éditorial
Publié
Date de publication
10/1985
Volume
162
Numéro
4
Pages
1393-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 1
Résumé
Three previously selected monoclonal antibodies (mAb) directed against the clonotypic structure of a variant (termed JA3) of the interleukin 2 (IL-2)-producing Jurkat leukemia cell line (anti-JTi1-3 mAb) were found to induce an adherent cell-dependent proliferation of peripheral blood T cells in 20 different donors. Unlike the early cell proliferation induced by anti-T3 mAb, anti-JTi mAb-induced proliferation was detectable at day 5-6 of culture and reached peak levels at day 7-9. Less than 1% JTi+ cells were consistently detected in the starting peripheral blood lymphocytes or in control cultures in which cells were stimulated with anti-T3, phytohemagglutinin, or allogeneic cells. However, JTi+ cells were found in increasing proportions after culture with anti-JTi mAb and they were mostly represented by large blast cells expressing either the T4 or the T8 antigen, together with typical activation antigens including HLA-DR, IL-2 receptor, and 4F2. Immunoprecipitation experiments and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that anti-JTi-reactive molecules present on antibody-stimulated lymphocytes or on JA3 cells were similar, disulphide-linked heterodimeric structures.
Mots-clé
Animals Antibodies, Monoclonal/*immunology Cell Line Leukemia, Experimental/immunology *Lymphocyte Activation Mice Molecular Weight Phenotype T-Lymphocytes/*immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:13
Dernière modification de la notice
20/08/2019 12:30
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