Differential messenger RNA distribution of lactate dehydrogenase LDH-1 and LDH-5 isoforms in the rat brain

Détails

ID Serval
serval:BIB_08088E48DB9A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differential messenger RNA distribution of lactate dehydrogenase LDH-1 and LDH-5 isoforms in the rat brain
Périodique
Neuroscience
Auteur⸱e⸱s
Laughton  J. D., Charnay  Y., Belloir  B., Pellerin  L., Magistretti  P. J., Bouras  C.
ISSN
0306-4522 (Print)
Statut éditorial
Publié
Date de publication
2000
Volume
96
Numéro
3
Pages
619-25
Notes
Journal Article
Résumé
The role of lactate in brain energy metabolism has recently received renewed attention. Although blood-borne monocarboxylates such as lactate poorly cross the blood-brain barrier in the adult brain, lactate produced within the brain parenchyma may be a suitable substrate for brain cells. Lactate dehydrogenase is crucial for both the production and utilization of lactate. In this article, we report the regional distribution of the messenger RNAs for lactate dehydrogenase isoforms 1 and 5 in the adult rat brain using in situ hybridization histochemistry with specific [alpha-(35)S]dATP 3' end-labeled oligoprobes. The autoradiographs revealed that the lactate dehydrogenase-1 messenger RNA is highly expressed in a variety of brain structures, including the main olfactory bulb, the piriform cortex, several thalamic and hypothalamic nuclei, the pontine nuclei, the ventral cochlear nucleus, the trigeminal nerve and the solitary tractus nucleus. In addition, the granular and Purkinje cell layers of the cerebellum showed a strong labeling. The neocortex (e.g., cingular, retrosplenial and frontoparietal cortices) often exhibits a marked laminar pattern of distribution of lactate dehydrogenase-1 messenger RNA (layers II/III, IV and VI being most strongly labeled). In contrast, expression of the lactate dehydrogenase-5 messenger RNA generally seemed more diffusely distributed across the different brain regions. Expression was particularly strong in the hippocampal formation (especially in Ammon's horn and dentate gyrus) and in the cerebral cortex, where no laminar pattern of distribution was observed. Overall, these data are consistent with the emerging idea that lactate is an important energy substrate produced and consumed by brain cells.
Mots-clé
Animals Autoradiography Brain/*enzymology Histocytochemistry In Situ Hybridization Isoenzymes L-Lactate Dehydrogenase/*genetics/metabolism Male RNA, Messenger/*metabolism Rats Rats, Wistar Tissue Distribution
Pubmed
Web of science
Création de la notice
24/01/2008 13:17
Dernière modification de la notice
20/08/2019 12:30
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